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Dynamic reconfiguration of subcompartment architectures in artificial cells.

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Title: Dynamic reconfiguration of subcompartment architectures in artificial cells.
Authors: Zubaite, G
Hindley, JW
Ces, O
Elani, Y
Item Type: Journal Article
Abstract: Artificial cells are minimal structures constructed from biomolecular building blocks designed to mimic cellular processes, behaviors, and architectures. One near-ubiquitous feature of cellular life is the spatial organization of internal content. We know from biology that organization of content (including in membrane-bound organelles) is linked to cellular functions and that this feature is dynamic: the presence, location, and degree of compartmentalization changes over time. Vesicle-based artificial cells, however, are not currently able to mimic this fundamental cellular property. Here, we describe an artificial cell design strategy that addresses this technological bottleneck. We create a series of artificial cell architectures which possess multicompartment assemblies localized either on the inner or on the outer surface of the artificial cell membrane. Exploiting liquid-liquid phase separation, we can also engineer spatially segregated regions of condensed subcompartments attached to the cell surface, aligning with coexisting membrane domains. These structures can sense changes in environmental conditions and respond by reversibly transitioning from condensed multicompartment layers on the membrane surface to a dispersed state in the cell lumen, mimicking the dynamic compartmentalization found in biological cells. Likewise, we engineer exosome-like subcompartments that can be released to the environment. We can achieve this by using two types of triggers: chemical (addition of salts) and mechanical (by pulling membrane tethers using optical traps). These approaches allow us to control the compartmentalization state of artificial cells on population and single-cell levels.
Issue Date: 13-Jun-2022
Date of Acceptance: 27-Apr-2022
URI: http://hdl.handle.net/10044/1/97452
DOI: 10.1021/acsnano.2c02195
ISSN: 1936-0851
Publisher: American Chemical Society
Journal / Book Title: ACS Nano
Volume: 16
Issue: 6
Copyright Statement: © 2022 The Authors. Published by American Chemical Society. This work is published under CC BY 4.0 International licence.
Sponsor/Funder: Medical Research Council
Engineering and Physical Sciences Research Council
Biotechnology and Biological Sciences Research Cou
Funder's Grant Number: MR/S031537/1
EP/V048651/1
BB/W00125X/1
Keywords: Science & Technology
Physical Sciences
Technology
Chemistry, Multidisciplinary
Chemistry, Physical
Nanoscience & Nanotechnology
Materials Science, Multidisciplinary
Chemistry
Science & Technology - Other Topics
Materials Science
artificial cells
phospholipids
compartments
organelles
vesicles
GIANT UNILAMELLAR VESICLES
MEMBRANE-FUSION
CALCIUM
COMPARTMENTALIZATION
ORGANIZATION
artificial cells
compartments
organelles
phospholipids
vesicles
artificial cells
compartments
organelles
phospholipids
vesicles
Nanoscience & Nanotechnology
Publication Status: Published online
Conference Place: United States
Open Access location: https://pubs.acs.org/doi/full/10.1021/acsnano.2c02195
Online Publication Date: 2022-06-13
Appears in Collections:Chemistry
Biological and Biophysical Chemistry
Chemical Engineering
Faculty of Natural Sciences
Faculty of Engineering



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