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1H NMR spectroscopy-based metabolomics analysis for the diagnosis of symptomatic E. coli-associated urinary tract infection (UTI)
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1H NMR spectroscopy-based metabolomics analysis for the diagnosis.pdf | Published version | 624.49 kB | Adobe PDF | View/Open |
Title: | 1H NMR spectroscopy-based metabolomics analysis for the diagnosis of symptomatic E. coli-associated urinary tract infection (UTI) |
Authors: | Lussu, M Camboni, T Piras, C Serra, C Del Carratore, F Griffin, J Atzori, L Manzin, A |
Item Type: | Journal Article |
Abstract: | BACKGROUND: Urinary tract infection (UTI) is one of the most common diagnoses in girls and women, and to a lesser extent in boys and men younger than 50 years. Escherichia coli, followed by Klebsiella spp. and Proteus spp., cause 75-90% of all infections. Infection of the urinary tract is identified by growth of a significant number of a single species in the urine, in the presence of symptoms. Urinary culture is an accurate diagnostic method but takes several hours or days to be carried out. Metabolomics analysis aims to identify biomarkers that are capable of speeding up diagnosis. METHODS: Urine samples from 51 patients with a prior diagnosis of Escherichia coli-associated UTI, from 21 patients with UTI caused by other pathogens (bacteria and fungi), and from 61 healthy controls were analyzed. The 1H-NMR spectra were acquired and processed. Multivariate statistical models were applied and their performance was validated using permutation test and ROC curve. RESULTS: Orthogonal Partial Least Squares-discriminant Analysis (OPLS-DA) showed good separation (R2Y = 0.76, Q2=0.45, p < 0.001) between UTI caused by Escherichia coli and healthy controls. Acetate and trimethylamine were identified as discriminant metabolites. The concentrations of both metabolites were calculated and used to build the ROC curves. The discriminant metabolites identified were also evaluated in urine samples from patients with other pathogens infections to test their specificity. CONCLUSIONS: Acetate and trimethylamine were identified as optimal candidates for biomarkers for UTI diagnosis. The conclusions support the possibility of a fast diagnostic test for Escherichia coli-associated UTI using acetate and trimethylamine concentrations. |
Issue Date: | 21-Sep-2017 |
Date of Acceptance: | 13-Sep-2017 |
URI: | http://hdl.handle.net/10044/1/75297 |
DOI: | 10.1186/s12866-017-1108-1 |
ISSN: | 1471-2180 |
Publisher: | BioMed Central |
Journal / Book Title: | BMC Microbiology |
Volume: | 17 |
Issue: | 1 |
Copyright Statement: | © The Author(s). 2017. This article is distributed under the terms of the Creative Commons Attribution 4.0International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, andreproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link tothe Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver(http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
Keywords: | 1H NMR spectroscopy Acetate E. coli ROC curve Trimethylamine UTI Acetates Adult Aged Aged, 80 and over Bacteria Bacteriuria Biomarkers Escherichia coli Escherichia coli Infections Female Fungi Humans Male Metabolomics Methylamines Middle Aged Multivariate Analysis Proton Magnetic Resonance Spectroscopy ROC Curve Reference Values Sensitivity and Specificity Time Factors Urinary Tract Urinary Tract Infections Urinary Tract Humans Bacteria Escherichia coli Fungi Escherichia coli Infections Urinary Tract Infections Bacteriuria Methylamines Acetates Multivariate Analysis Sensitivity and Specificity ROC Curve Time Factors Reference Values Adult Aged Aged, 80 and over Middle Aged Female Male Metabolomics Proton Magnetic Resonance Spectroscopy Biomarkers 1H NMR spectroscopy Acetate E. coli ROC curve Trimethylamine UTI Acetates Adult Aged Aged, 80 and over Bacteria Bacteriuria Biomarkers Escherichia coli Escherichia coli Infections Female Fungi Humans Male Metabolomics Methylamines Middle Aged Multivariate Analysis Proton Magnetic Resonance Spectroscopy ROC Curve Reference Values Sensitivity and Specificity Time Factors Urinary Tract Urinary Tract Infections 06 Biological Sciences 11 Medical and Health Sciences 07 Agricultural and Veterinary Sciences Microbiology |
Publication Status: | Published |
Conference Place: | England |
Article Number: | ARTN 201 |
Appears in Collections: | Department of Metabolism, Digestion and Reproduction |