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A highly sensitive molecular structural probe applied to in-situ biosensing of metabolites using PEDOT:PSS
File | Description | Size | Format | |
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ET_manuscript_BiotechandBioeng_accepted.pdf | Accepted version | 175.99 kB | Adobe PDF | View/Open |
Figures_accepted.pdf | Accepted version | 398.15 kB | Adobe PDF | View/Open |
Title: | A highly sensitive molecular structural probe applied to in-situ biosensing of metabolites using PEDOT:PSS |
Authors: | Tan, E Pappa, A-M Pitsalidis, C Nightingale, J Wood, S Castro, FA Owens, RM Kim, J-S |
Item Type: | Journal Article |
Abstract: | A large amount of research within organic biosensors is dominated by organic electrochemical transistors (OECTs) that use conducting polymers such as poly(3,4-ethylene dioxythiophene doped with poly(styrenesulfonate) (PEDOT:PSS). Despite the recent advances in OECT-based biosensors, the sensing is solely reliant on the amperometric detection of the bioanalytes. This is typically accompanied by large undesirable parasitic electrical signals from the electroactive components in the electrolyte. Herein, we present the use of in-situ resonance Raman spectroscopy to probe subtle molecular structural changes of PEDOT:PSS associated with its doping level. We demonstrate how such doping level changes of PEDOT:PSS can be used, for the first time, on operational OECTs for sensitive and selective metabolite sensing whilst simultaneously performing amperometric detection of the analyte. We test the sensitivity by molecularly sensing a lowest glucose concentration of 0.02 mM in phosphate buffered saline (PBS) solution. By changing the electrolyte to cell culture media, the selectivity of in-situ resonance Raman spectroscopy is emphasized as it remains unaffected by other electroactive components in the electrolyte. The application of this molecular structural probe highlights the importance of developing biosensing probes that benefit from high sensitivity of the material's structural and electrical properties whilst being complimentary with the electronic methods of detection. |
Issue Date: | Jan-2020 |
Date of Acceptance: | 4-Oct-2019 |
URI: | http://hdl.handle.net/10044/1/74126 |
DOI: | 10.1002/bit.27187 |
ISSN: | 0006-3592 |
Publisher: | Wiley |
Start Page: | 291 |
End Page: | 299 |
Journal / Book Title: | Biotechnology and Bioengineering |
Volume: | 117 |
Issue: | 1 |
Copyright Statement: | © 2019 Owner. This is the accepted version of the following article: Tan, E, Pappa, A‐M, Pitsalidis, C, et al. A highly sensitive molecular structural probe applied to in situ biosensing of metabolites using PEDOT:PSS. Biotechnology and Bioengineering. 2019; 1– 9. https://doi.org/10.1002/bit.27187, which has been published in final form at https://doi.org/10.1002/bit.27187. |
Sponsor/Funder: | Engineering and Physical Sciences Research Council |
Funder's Grant Number: | EP/L016702/1 |
Keywords: | Science & Technology Life Sciences & Biomedicine Biotechnology & Applied Microbiology doping level in situ metabolite sensing OECT PEDOT:PSS resonance Raman ORGANIC ELECTROCHEMICAL TRANSISTORS LIGHT-EMITTING-DIODES RAMAN POLYMER POLY(3,4-ETHYLENEDIOXYTHIOPHENE) TRANSCONDUCTANCE OECT PEDOT:PSS doping level in situ metabolite sensing resonance Raman OECT PEDOT:PSS doping level in-situ metabolite sensing resonance Raman Biotechnology |
Publication Status: | Published |
Conference Place: | United States |
Online Publication Date: | 2019-10-07 |
Appears in Collections: | Physics Experimental Solid State Faculty of Natural Sciences |