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A highly sensitive molecular structural probe applied to in-situ biosensing of metabolites using PEDOT:PSS

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Title: A highly sensitive molecular structural probe applied to in-situ biosensing of metabolites using PEDOT:PSS
Authors: Tan, E
Pappa, A-M
Pitsalidis, C
Nightingale, J
Wood, S
Castro, FA
Owens, RM
Kim, J-S
Item Type: Journal Article
Abstract: A large amount of research within organic biosensors is dominated by organic electrochemical transistors (OECTs) that use conducting polymers such as poly(3,4-ethylene dioxythiophene doped with poly(styrenesulfonate) (PEDOT:PSS). Despite the recent advances in OECT-based biosensors, the sensing is solely reliant on the amperometric detection of the bioanalytes. This is typically accompanied by large undesirable parasitic electrical signals from the electroactive components in the electrolyte. Herein, we present the use of in-situ resonance Raman spectroscopy to probe subtle molecular structural changes of PEDOT:PSS associated with its doping level. We demonstrate how such doping level changes of PEDOT:PSS can be used, for the first time, on operational OECTs for sensitive and selective metabolite sensing whilst simultaneously performing amperometric detection of the analyte. We test the sensitivity by molecularly sensing a lowest glucose concentration of 0.02 mM in phosphate buffered saline (PBS) solution. By changing the electrolyte to cell culture media, the selectivity of in-situ resonance Raman spectroscopy is emphasized as it remains unaffected by other electroactive components in the electrolyte. The application of this molecular structural probe highlights the importance of developing biosensing probes that benefit from high sensitivity of the material's structural and electrical properties whilst being complimentary with the electronic methods of detection.
Issue Date: Jan-2020
Date of Acceptance: 4-Oct-2019
URI: http://hdl.handle.net/10044/1/74126
DOI: 10.1002/bit.27187
ISSN: 0006-3592
Publisher: Wiley
Start Page: 291
End Page: 299
Journal / Book Title: Biotechnology and Bioengineering
Volume: 117
Issue: 1
Copyright Statement: © 2019 Owner. This is the accepted version of the following article: Tan, E, Pappa, A‐M, Pitsalidis, C, et al. A highly sensitive molecular structural probe applied to in situ biosensing of metabolites using PEDOT:PSS. Biotechnology and Bioengineering. 2019; 1– 9. https://doi.org/10.1002/bit.27187, which has been published in final form at https://doi.org/10.1002/bit.27187.
Sponsor/Funder: Engineering and Physical Sciences Research Council
Funder's Grant Number: EP/L016702/1
Keywords: Science & Technology
Life Sciences & Biomedicine
Biotechnology & Applied Microbiology
doping level
in situ
metabolite sensing
OECT
PEDOT:PSS
resonance Raman
ORGANIC ELECTROCHEMICAL TRANSISTORS
LIGHT-EMITTING-DIODES
RAMAN
POLYMER
POLY(3,4-ETHYLENEDIOXYTHIOPHENE)
TRANSCONDUCTANCE
OECT
PEDOT:PSS
doping level
in situ
metabolite sensing
resonance Raman
OECT
PEDOT:PSS
doping level
in-situ
metabolite sensing
resonance Raman
Biotechnology
Publication Status: Published
Conference Place: United States
Online Publication Date: 2019-10-07
Appears in Collections:Physics
Experimental Solid State
Faculty of Natural Sciences