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A bacteriophage transcription regulator inhibits bacterial transcription initiation by Sigma-factor displacement

Title: A bacteriophage transcription regulator inhibits bacterial transcription initiation by Sigma-factor displacement
Authors: Liu, B
Shadrin, A
Sheppard, C
Mekler, V
Xu, Y
Severinov, K
Matthews, S
Wigneshweraraj, S
Item Type: Journal Article
Abstract: Bacteriophages (phages) appropriate essential processes of bacterial hosts to benefit their own development. The multisubunit bacterial RNA polymerase (RNAp) enzyme, which catalyses DNA transcription, is targeted by phage-encoded transcription regulators that selectively modulate its activity. Here, we describe the structural and mechanistic basis for the inhibition of bacterial RNAp by the transcription regulator P7 encoded by Xanthomonas oryzae phage Xp10. We reveal that P7 uses a two-step mechanism to simultaneously interact with the catalytic β and β’ subunits of the bacterial RNAp and inhibits transcription initiation by inducing the displacement of the σ70-factor on initial engagement of RNAp with promoter DNA. The new mode of interaction with and inhibition mechanism of bacterial RNAp by P7 underscore the remarkable variety of mechanisms evolved by phages to interfere with host transcription.
Issue Date: 1-Apr-2014
Date of Acceptance: 30-Dec-2013
URI: http://hdl.handle.net/10044/1/17928
DOI: 10.1093/nar/gku080
ISSN: 0305-1048
Publisher: Oxford University Press
Start Page: 4294
End Page: 4305
Journal / Book Title: Nucleic Acids Research
Volume: 42
Issue: 7
Copyright Statement: (c) The Author(s) 2014. Published by Oxford University Press.
Sponsor/Funder: Biotechnology and Biological Sciences Research Council (BBSRC)
Medical Research Council (MRC)
Funder's Grant Number: BB/K000233/1
G1001664
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
COLI RNA-POLYMERASE
BETA-FLAP DOMAIN
CRYSTAL-STRUCTURE
PROTEIN CONTACTS
GENE-EXPRESSION
PROMOTER
SUBUNIT
TERMINATION
RESONANCE
RESIDUES
Bacteriophages
DNA-Directed RNA Polymerases
Models, Molecular
Protein Structure, Tertiary
Protein Subunits
Sigma Factor
Transcription Factors
Transcription Initiation, Genetic
Viral Proteins
Bacteriophages
DNA-Directed RNA Polymerases
Protein Subunits
Transcription Factors
Sigma Factor
Viral Proteins
Protein Structure, Tertiary
Models, Molecular
Transcription Initiation, Genetic
Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
COLI RNA-POLYMERASE
BETA-FLAP DOMAIN
CRYSTAL-STRUCTURE
PROTEIN CONTACTS
GENE-EXPRESSION
PROMOTER
SUBUNIT
TERMINATION
RESONANCE
RESIDUES
Developmental Biology
05 Environmental Sciences
06 Biological Sciences
08 Information and Computing Sciences
Publication Status: Published
Online Publication Date: 2014-01-28
Appears in Collections:Department of Infectious Diseases
Faculty of Medicine
Faculty of Natural Sciences