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A bacteriophage transcription regulator inhibits bacterial transcription initiation by Sigma-factor displacement
File | Description | Size | Format | |
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A bacteriophage transcription regulator inhibits bacterial transcription initiation by σ-factor displacement..pdf | Accepted version | 5.66 MB | Adobe PDF | View/Open |
Title: | A bacteriophage transcription regulator inhibits bacterial transcription initiation by Sigma-factor displacement |
Authors: | Liu, B Shadrin, A Sheppard, C Mekler, V Xu, Y Severinov, K Matthews, S Wigneshweraraj, S |
Item Type: | Journal Article |
Abstract: | Bacteriophages (phages) appropriate essential processes of bacterial hosts to benefit their own development. The multisubunit bacterial RNA polymerase (RNAp) enzyme, which catalyses DNA transcription, is targeted by phage-encoded transcription regulators that selectively modulate its activity. Here, we describe the structural and mechanistic basis for the inhibition of bacterial RNAp by the transcription regulator P7 encoded by Xanthomonas oryzae phage Xp10. We reveal that P7 uses a two-step mechanism to simultaneously interact with the catalytic β and β’ subunits of the bacterial RNAp and inhibits transcription initiation by inducing the displacement of the σ70-factor on initial engagement of RNAp with promoter DNA. The new mode of interaction with and inhibition mechanism of bacterial RNAp by P7 underscore the remarkable variety of mechanisms evolved by phages to interfere with host transcription. |
Issue Date: | 1-Apr-2014 |
Date of Acceptance: | 30-Dec-2013 |
URI: | http://hdl.handle.net/10044/1/17928 |
DOI: | 10.1093/nar/gku080 |
ISSN: | 0305-1048 |
Publisher: | Oxford University Press |
Start Page: | 4294 |
End Page: | 4305 |
Journal / Book Title: | Nucleic Acids Research |
Volume: | 42 |
Issue: | 7 |
Copyright Statement: | (c) The Author(s) 2014. Published by Oxford University Press. |
Sponsor/Funder: | Biotechnology and Biological Sciences Research Council (BBSRC) Medical Research Council (MRC) |
Funder's Grant Number: | BB/K000233/1 G1001664 |
Keywords: | Science & Technology Life Sciences & Biomedicine Biochemistry & Molecular Biology COLI RNA-POLYMERASE BETA-FLAP DOMAIN CRYSTAL-STRUCTURE PROTEIN CONTACTS GENE-EXPRESSION PROMOTER SUBUNIT TERMINATION RESONANCE RESIDUES Bacteriophages DNA-Directed RNA Polymerases Models, Molecular Protein Structure, Tertiary Protein Subunits Sigma Factor Transcription Factors Transcription Initiation, Genetic Viral Proteins Bacteriophages DNA-Directed RNA Polymerases Protein Subunits Transcription Factors Sigma Factor Viral Proteins Protein Structure, Tertiary Models, Molecular Transcription Initiation, Genetic Science & Technology Life Sciences & Biomedicine Biochemistry & Molecular Biology COLI RNA-POLYMERASE BETA-FLAP DOMAIN CRYSTAL-STRUCTURE PROTEIN CONTACTS GENE-EXPRESSION PROMOTER SUBUNIT TERMINATION RESONANCE RESIDUES Developmental Biology 05 Environmental Sciences 06 Biological Sciences 08 Information and Computing Sciences |
Publication Status: | Published |
Online Publication Date: | 2014-01-28 |
Appears in Collections: | Department of Infectious Diseases Faculty of Medicine Faculty of Natural Sciences |