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3D printed silica-gelatin hybrid scaffolds of specific channel sizes promote collagen Type II, Sox9 and Aggrecan production from chondrocytes
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Nelson et al accepted.pdf | Accepted version | 9.08 MB | Adobe PDF | View/Open |
Title: | 3D printed silica-gelatin hybrid scaffolds of specific channel sizes promote collagen Type II, Sox9 and Aggrecan production from chondrocytes |
Authors: | Nelson, M Li, S Page, SJ Shi, X Lee, PD Stevens, MM Hanna, JV Jones, JR |
Item Type: | Journal Article |
Abstract: | Inorganic/organic hybrids have co-networks of inorganic and organic components, with the aim of obtaining synergy of the properties of those components. Here, a silica-gelatin sol-gel hybrid “ink” was directly 3D printed to produce 3D grid-like scaffolds, using a coupling agent, 3-glycidyloxypropyl)trimethoxysilane (GPTMS), to form covalent bonds between the silicate and gelatin co-networks. Scaffolds were printed with 1 mm strut separation, but the drying method affected the final architecture and properties. Freeze drying produced <40 μm struts and large ~700 μm channels. Critical point drying enabled strut consolidation, with ~160 μm struts and ~200 μm channels, which improved mechanical properties. This architecture was critical to cellular response: when chondrocytes were seeded on the scaffolds with 200 μm wide pore channels in vitro, collagen Type II matrix was preferentially produced (negligible amount of Type I or X were observed), indicative of hyaline-like cartilaginous matrix formation, but when pore channels were 700 μm wide, Type I collagen was prevalent. This was supported by Sox9 and Aggrecan expression. The scaffolds have potential for regeneration of articular cartilage regeneration, particularly in sports medicine cases. |
Issue Date: | Apr-2021 |
Date of Acceptance: | 8-Feb-2021 |
URI: | http://hdl.handle.net/10044/1/87536 |
DOI: | 10.1016/j.msec.2021.111964 |
ISSN: | 0928-4931 |
Publisher: | Elsevier BV |
Start Page: | 1 |
End Page: | 12 |
Journal / Book Title: | Materials Science and Engineering: C |
Volume: | 123 |
Copyright Statement: | © 2021 Elsevier Ltd. All rights reserved. This manuscript is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence http://creativecommons.org/licenses/by-nc-nd/4.0/ |
Sponsor/Funder: | Engineering & Physical Science Research Council (EPSRC) Medical Research Council (MRC) |
Funder's Grant Number: | EP/I020861/1 MR/R015651/1 |
Keywords: | 3D printing Articular cartilage Hybrid Scaffold Biomedical Engineering 0903 Biomedical Engineering 0912 Materials Engineering |
Publication Status: | Published |
Article Number: | 111964 |
Online Publication Date: | 2021-02-12 |
Appears in Collections: | Materials Faculty of Natural Sciences |
This item is licensed under a Creative Commons License