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3D printed silica-gelatin hybrid scaffolds of specific channel sizes promote collagen Type II, Sox9 and Aggrecan production from chondrocytes

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Title: 3D printed silica-gelatin hybrid scaffolds of specific channel sizes promote collagen Type II, Sox9 and Aggrecan production from chondrocytes
Authors: Nelson, M
Li, S
Page, SJ
Shi, X
Lee, PD
Stevens, MM
Hanna, JV
Jones, JR
Item Type: Journal Article
Abstract: Inorganic/organic hybrids have co-networks of inorganic and organic components, with the aim of obtaining synergy of the properties of those components. Here, a silica-gelatin sol-gel hybrid “ink” was directly 3D printed to produce 3D grid-like scaffolds, using a coupling agent, 3-glycidyloxypropyl)trimethoxysilane (GPTMS), to form covalent bonds between the silicate and gelatin co-networks. Scaffolds were printed with 1 mm strut separation, but the drying method affected the final architecture and properties. Freeze drying produced <40 μm struts and large ~700 μm channels. Critical point drying enabled strut consolidation, with ~160 μm struts and ~200 μm channels, which improved mechanical properties. This architecture was critical to cellular response: when chondrocytes were seeded on the scaffolds with 200 μm wide pore channels in vitro, collagen Type II matrix was preferentially produced (negligible amount of Type I or X were observed), indicative of hyaline-like cartilaginous matrix formation, but when pore channels were 700 μm wide, Type I collagen was prevalent. This was supported by Sox9 and Aggrecan expression. The scaffolds have potential for regeneration of articular cartilage regeneration, particularly in sports medicine cases.
Issue Date: Apr-2021
Date of Acceptance: 8-Feb-2021
URI: http://hdl.handle.net/10044/1/87536
DOI: 10.1016/j.msec.2021.111964
ISSN: 0928-4931
Publisher: Elsevier BV
Start Page: 1
End Page: 12
Journal / Book Title: Materials Science and Engineering: C
Volume: 123
Copyright Statement: © 2021 Elsevier Ltd. All rights reserved. This manuscript is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence http://creativecommons.org/licenses/by-nc-nd/4.0/
Sponsor/Funder: Engineering & Physical Science Research Council (EPSRC)
Medical Research Council (MRC)
Funder's Grant Number: EP/I020861/1
MR/R015651/1
Keywords: 3D printing
Articular cartilage
Hybrid
Scaffold
Biomedical Engineering
0903 Biomedical Engineering
0912 Materials Engineering
Publication Status: Published
Article Number: 111964
Online Publication Date: 2021-02-12
Appears in Collections:Materials
Faculty of Natural Sciences



This item is licensed under a Creative Commons License Creative Commons