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Zebrafish embryo xenograft and metastasis assay

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Title: Zebrafish embryo xenograft and metastasis assay
Authors: Paatero, I
Alve, S
Gramolelli, S
Ivaska, J
Ojala, PM
Item Type: Journal Article
Abstract: Xenograft models, and in particular the mouse xenograft model, where human cancer cells are transplanted into immunocompromised mice, have been used extensively in cancer studies. Although these models have contributed enormously to our understanding of cancer biology, the zebrafish xenograft model offers several advantages over the mouse model. Zebrafish embryos can be easily cultured in large quantities, are small and easy to handle, making it possible to use a high number of embryos for each experimental condition. Young embryos lack an efficient immune system. Therefore the injected cancer cells are not rejected, and the formation of primary tumors and micrometastases is rapid. Transparency of the embryos enables imaging of primary tumors and metastases in an intact and living embryo. Here we describe a method where GFP expressing tumor cells are injected into pericardial space of zebrafish embryos. At four days post-injection, the embryos are imaged and the formation of primary tumor and distant micrometastases are analyzed.
Issue Date: 20-Sep-2018
Date of Acceptance: 6-Sep-2018
URI: http://hdl.handle.net/10044/1/84633
DOI: 10.21769/BioProtoc.3027
ISSN: 2331-8325
Publisher: Bio-Protocol
Journal / Book Title: Bio-protocol
Volume: 8
Issue: 18
Copyright Statement: © Copyright Paateroet al. This article is distributed under the terms of the Creative Commons Attribution License (CC BY 4.0 ).
Keywords: Science & Technology
Life Sciences & Biomedicine
Biology
Life Sciences & Biomedicine - Other Topics
Zebrafish
Embryo
Cancer
Xenograft
Melanoma
Micrometastases
Protocol
CANCER
Science & Technology
Life Sciences & Biomedicine
Biology
Life Sciences & Biomedicine - Other Topics
Zebrafish
Embryo
Cancer
Xenograft
Melanoma
Micrometastases
Protocol
CANCER
Publication Status: Published
Article Number: ARTN e3027
Appears in Collections:Department of Infectious Diseases



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