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Serum exosomal miRNAs are associated with active pulmonary tuberculosis

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Title: Serum exosomal miRNAs are associated with active pulmonary tuberculosis
Authors: Alipoor, SD
Tabarsi, P
Varahram, M
Movassaghi, M
Dizaji, MK
Folkerts, G
Garssen, J
Adcock, IM
Mortaz, E
Item Type: Journal Article
Abstract: Introduction. Tuberculosis (TB) remains a major threat to human health. Due to the limited accuracy of the current TB diagnostic tests, it is critical to determine novel biomarkers for this disease. Circulating exosomes have been used as diagnostic biomarkers in various diseases. Objective of the Study. In this pilot study, we examined the expression of miRNAs as biomarker candidates for the diagnosis of TB infection. Methods. Serum-derived exosomes were isolated from TB patients and matched control subjects. The expression of miR-484, miR-425, and miR-96 was examined by RT-PCR methods. Results. The expression of miR-484, miR-425, and miR-96 were significantly increased in serum of TB patients which correlated with the TB infection level. A receiver operating characteristic (ROC) curve analysis showed the diagnostic potency of each individual serum exosomal miRNA with an area under the curve AUC = 0 72 for miR-484 (p < 0 05), 0.66 for miR-425 (p < 0 05), and 0.62 for miR-96 (p < 0 05). Conclusion. These results demonstrate that exosomal miRNAs have diagnostic potential in active tuberculosis. The diagnostic power may be improved when combined with conventional diagnostic markers.
Issue Date: 11-Feb-2019
Date of Acceptance: 5-Nov-2018
URI: http://hdl.handle.net/10044/1/77641
DOI: 10.1155/2019/1907426
ISSN: 0278-0240
Publisher: Hindawi Publishing Corporation
Journal / Book Title: Disease Markers
Volume: 2019
Copyright Statement: © 2019 Shamila D. Alipoor et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Keywords: Science & Technology
Life Sciences & Biomedicine
Biotechnology & Applied Microbiology
Genetics & Heredity
Medicine, Research & Experimental
Pathology
Research & Experimental Medicine
EXPRESSION
BIOMARKERS
CELLS
Adolescent
Adult
Biomarkers
Exosomes
Female
Humans
Male
MicroRNAs
Middle Aged
Tuberculosis, Pulmonary
Humans
Tuberculosis, Pulmonary
MicroRNAs
Adolescent
Adult
Middle Aged
Female
Male
Exosomes
Biomarkers
Science & Technology
Life Sciences & Biomedicine
Biotechnology & Applied Microbiology
Genetics & Heredity
Medicine, Research & Experimental
Pathology
Research & Experimental Medicine
EXPRESSION
BIOMARKERS
CELLS
1116 Medical Physiology
Oncology & Carcinogenesis
Publication Status: Published
Article Number: ARTN 1907426
Online Publication Date: 2019-02-11
Appears in Collections:National Heart and Lung Institute