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Serum exosomal miRNAs are associated with active pulmonary tuberculosis
File | Description | Size | Format | |
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1907426.pdf | Published version | 1.79 MB | Adobe PDF | View/Open |
Title: | Serum exosomal miRNAs are associated with active pulmonary tuberculosis |
Authors: | Alipoor, SD Tabarsi, P Varahram, M Movassaghi, M Dizaji, MK Folkerts, G Garssen, J Adcock, IM Mortaz, E |
Item Type: | Journal Article |
Abstract: | Introduction. Tuberculosis (TB) remains a major threat to human health. Due to the limited accuracy of the current TB diagnostic tests, it is critical to determine novel biomarkers for this disease. Circulating exosomes have been used as diagnostic biomarkers in various diseases. Objective of the Study. In this pilot study, we examined the expression of miRNAs as biomarker candidates for the diagnosis of TB infection. Methods. Serum-derived exosomes were isolated from TB patients and matched control subjects. The expression of miR-484, miR-425, and miR-96 was examined by RT-PCR methods. Results. The expression of miR-484, miR-425, and miR-96 were significantly increased in serum of TB patients which correlated with the TB infection level. A receiver operating characteristic (ROC) curve analysis showed the diagnostic potency of each individual serum exosomal miRNA with an area under the curve AUC = 0 72 for miR-484 (p < 0 05), 0.66 for miR-425 (p < 0 05), and 0.62 for miR-96 (p < 0 05). Conclusion. These results demonstrate that exosomal miRNAs have diagnostic potential in active tuberculosis. The diagnostic power may be improved when combined with conventional diagnostic markers. |
Issue Date: | 11-Feb-2019 |
Date of Acceptance: | 5-Nov-2018 |
URI: | http://hdl.handle.net/10044/1/77641 |
DOI: | 10.1155/2019/1907426 |
ISSN: | 0278-0240 |
Publisher: | Hindawi Publishing Corporation |
Journal / Book Title: | Disease Markers |
Volume: | 2019 |
Copyright Statement: | © 2019 Shamila D. Alipoor et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
Keywords: | Science & Technology Life Sciences & Biomedicine Biotechnology & Applied Microbiology Genetics & Heredity Medicine, Research & Experimental Pathology Research & Experimental Medicine EXPRESSION BIOMARKERS CELLS Adolescent Adult Biomarkers Exosomes Female Humans Male MicroRNAs Middle Aged Tuberculosis, Pulmonary Humans Tuberculosis, Pulmonary MicroRNAs Adolescent Adult Middle Aged Female Male Exosomes Biomarkers Science & Technology Life Sciences & Biomedicine Biotechnology & Applied Microbiology Genetics & Heredity Medicine, Research & Experimental Pathology Research & Experimental Medicine EXPRESSION BIOMARKERS CELLS 1116 Medical Physiology Oncology & Carcinogenesis |
Publication Status: | Published |
Article Number: | ARTN 1907426 |
Online Publication Date: | 2019-02-11 |
Appears in Collections: | National Heart and Lung Institute |