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A sensitised RNAi screen reveals a ch-TOG genetic interaction network required for spindle assembly
| File | Description | Size | Format | |
|---|---|---|---|---|
 A sensitised RNAi screen reveals a ch-TOG genetic interaction network required for spindle assembly.pdf | Published version | 2.63 MB | Adobe PDF | View/Open |
| Title: | A sensitised RNAi screen reveals a ch-TOG genetic interaction network required for spindle assembly |
| Authors: | Barr, AR Bakal, C |
| Item Type: | Journal Article |
| Abstract: | How multiple spindle assembly pathways are integrated to drive bipolar spindle assembly is poorly understood. We performed an image-based double RNAi screen to identify genes encoding Microtubule-Associated Proteins (MAPs) that interact with the highly conserved ch-TOG gene to regulate bipolar spindle assembly in human cells. We identified a ch-TOG centred network of genetic interactions which promotes centrosome-mediated microtubule polymerisation, leading to the incorporation of microtubules polymerised by all pathways into a bipolar structure. Our genetic screen also reveals that ch-TOG maintains a dynamic microtubule population, in part, through modulating HSET activity. ch-TOG ensures that spindle assembly is robust to perturbation but sufficiently dynamic such that spindles can explore a diverse shape space in search of structures that can align chromosomes. |
| Issue Date: | 3-Jun-2015 |
| Date of Acceptance: | 20-Apr-2015 |
| URI: | http://hdl.handle.net/10044/1/70558 |
| DOI: | https://doi.org/10.1038/srep10564 |
| ISSN: | 2045-2322 |
| Publisher: | Nature Publishing Group |
| Journal / Book Title: | Scientific Reports |
| Volume: | 5 |
| Copyright Statement: | © 2015 Authors. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| Keywords: | Science & Technology Multidisciplinary Sciences Science & Technology - Other Topics XENOPUS EGG EXTRACTS MICROTUBULE-ASSOCIATED PROTEIN CROSS-LINKS MICROTUBULES SISTER-CHROMATID COHESION MITOTIC-SPINDLE PLUS-END INTERACTION MAP CELL-DIVISION CHROMOSOME CONGRESSION SIGNALING NETWORKS Chromatin Cluster Analysis Dyneins Epistasis, Genetic Gene Expression Profiling Gene Regulatory Networks Humans Kinesin Microtubule-Associated Proteins Microtubules Mitosis Protein Binding RNA Interference RNA, Small Interfering Spindle Apparatus Tubulin Chromatin Microtubules Humans Tubulin Kinesin Microtubule-Associated Proteins RNA, Small Interfering Cluster Analysis Gene Expression Profiling Mitosis Epistasis, Genetic RNA Interference Protein Binding Gene Regulatory Networks Dyneins Spindle Apparatus Science & Technology Multidisciplinary Sciences Science & Technology - Other Topics XENOPUS EGG EXTRACTS MICROTUBULE-ASSOCIATED PROTEIN CROSS-LINKS MICROTUBULES SISTER-CHROMATID COHESION MITOTIC-SPINDLE PLUS-END INTERACTION MAP CELL-DIVISION CHROMOSOME CONGRESSION SIGNALING NETWORKS |
| Publication Status: | Published |
| Article Number: | 10564 |
| Online Publication Date: | 2015-06-03 |
| Appears in Collections: | Institute of Clinical Sciences |