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Identification of potential biomarkers of vaccine inflammation in mice

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Title: Identification of potential biomarkers of vaccine inflammation in mice
Authors: McKay, P
Cizmeci, D
Aldon, Y
Maertzdorf, J
Weiner, J
Kaufmann, S
Lewis, D
Van den Berg, R
Del Giudice, G
Shattock, R
Item Type: Journal Article
Abstract: Systems vaccinology approaches have been used successfully to define early signatures of the vaccine-induced immune response. However, the possibility that transcriptomics can also identify a correlate or surrogate for vaccine inflammation has not been fully explored. We have compared four licensed vaccines with known safety profiles, as well as three agonists of Toll-like receptors (TLRs) with known inflammatory potential, to elucidate the transcriptomic profile of an acceptable response to vaccination versus that of an inflammatory reaction. In mice, we looked at the transcriptomic changes in muscle at the injection site, the lymph node that drained the muscle, and the peripheral blood mononuclear cells (PBMCs)isolated from the circulating blood from 4 hr after injection and over the next week. A detailed examination and comparative analysis of these transcriptomes revealed a set of novel biomarkers that are reflective of inflammation after vaccination. These biomarkers are readily measurable in the peripheral blood, providing useful surrogates of inflammation, and provide a way to select candidates with acceptable safety profiles.
Issue Date: 14-May-2019
Date of Acceptance: 14-May-2019
URI: http://hdl.handle.net/10044/1/70552
DOI: https://dx.doi.org/10.7554/eLife.46149
ISSN: 2050-084X
Publisher: eLife Sciences Publications Ltd
Journal / Book Title: eLife
Volume: 8
Copyright Statement: Copyright © 2019 McKay et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Sponsor/Funder: Commission of the European Communities
Funder's Grant Number: 115308
Keywords: immunology
inflammation
mouse
Publication Status: Published
Article Number: e46149
Appears in Collections:Department of Medicine (up to 2019)