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A dual tracer F-18-FCH/F-18-FDG PET imaging of an orthotopic brain tumor xenograft model

Title: A dual tracer F-18-FCH/F-18-FDG PET imaging of an orthotopic brain tumor xenograft model
Authors: Fu, Y
Ong, L-C
Ranganath, SH
Zheng, L
Kee, I
Zhan, W
Yu, S
Chow, PKH
Wang, C-H
Item Type: Journal Article
Abstract: Early diagnosis of low grade glioma has been a challenge to clinicians. Positron Emission Tomography (PET) using 18F-FDG as a radio-tracer has limited utility in this area because of the high background in normal brain tissue. Other radiotracers such as 18F-Fluorocholine (18FFCH) could provide better contrast between tumor and normal brain tissue but with high incidence of false positives. In this study, the potential application of a dual tracer 18F-FCH/18FFDG-PET is investigated in order to improve the sensitivity of PET imaging for low grade glioma diagnosis based on a mouse orthotopic xenograft model. BALB/c nude mice with and without orthotopic glioma xenografts from U87 MG-luc2 glioma cell line are used for the study. The animals are subjected to 18F-FCH and 18F-FDG PET imaging, and images acquired from two separate scans are superimposed for analysis. The 18F-FCH counts are subtracted from the merged images to identify the tumor. Micro-CT, bioluminescence imaging (BLI), histology and measurement of the tumor diameter are also conducted for comparison. Results show that there is a significant contrast in 18F-FCH uptake between tumor and normal brain tissue (2.65 ± 0.98), but with a high false positive rate of 28.6%. The difficulty of identifying the tumor by 18FFDG only is also proved in this study. All the tumors can be detected based on the dual tracer technique of 18F-FCH/ 18F-FDG-PET imaging in this study, while the false-positive caused by 18F-FCH can be eliminated. Dual tracer 18F-FCH/18F-FDG PET imaging has the potential to improve the visualization of low grade glioma. 18F-FCH delineates tumor areas and the tumor can be identified by subtracting the 18F-FCH counts. The sensitivity was over 95%. Further studies are required to evaluate the possibility of applying this technique in clinical trials.
Issue Date: 4-Feb-2016
Date of Acceptance: 13-Jan-2016
URI: http://hdl.handle.net/10044/1/69145
DOI: https://doi.org/10.1371/journal.pone.0148123
ISSN: 1932-6203
Publisher: Public Library of Science (PLoS)
Journal / Book Title: PLoS ONE
Volume: 11
Issue: 2
Copyright Statement: © 2016 Fu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
POSITRON-EMISSION-TOMOGRAPHY
MINIMALLY INVASIVE SURGERY
RADIATION-DOSE ESCALATION
C-11 CHOLINE PET
GLIOBLASTOMA-MULTIFORME
FOLLOW-UP
F-18-FLUOROCHOLINE
PROLIFERATION
F-18-FDG
GLIOMAS
Animals
Brain Neoplasms
Cell Line, Tumor
Choline
Disease Models, Animal
Fluorodeoxyglucose F18
Glioblastoma
Heterografts
Humans
Image Processing, Computer-Assisted
Luminescent Measurements
Mice
Positron-Emission Tomography
Radiopharmaceuticals
General Science & Technology
MD Multidisciplinary
Publication Status: Published
Article Number: e0148123
Online Publication Date: 2016-02-04
Appears in Collections:Mechanical Engineering