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A dual tracer F-18-FCH/F-18-FDG PET imaging of an orthotopic brain tumor xenograft model
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A Dual Tracer 18F-FCH18F-FDG PET Imaging of an Orthotopic Brain Tumor Xenograft Model.pdf | Published version | 8.19 MB | Adobe PDF | View/Open |
Title: | A dual tracer F-18-FCH/F-18-FDG PET imaging of an orthotopic brain tumor xenograft model |
Authors: | Fu, Y Ong, L-C Ranganath, SH Zheng, L Kee, I Zhan, W Yu, S Chow, PKH Wang, C-H |
Item Type: | Journal Article |
Abstract: | Early diagnosis of low grade glioma has been a challenge to clinicians. Positron Emission Tomography (PET) using 18F-FDG as a radio-tracer has limited utility in this area because of the high background in normal brain tissue. Other radiotracers such as 18F-Fluorocholine (18FFCH) could provide better contrast between tumor and normal brain tissue but with high incidence of false positives. In this study, the potential application of a dual tracer 18F-FCH/18FFDG-PET is investigated in order to improve the sensitivity of PET imaging for low grade glioma diagnosis based on a mouse orthotopic xenograft model. BALB/c nude mice with and without orthotopic glioma xenografts from U87 MG-luc2 glioma cell line are used for the study. The animals are subjected to 18F-FCH and 18F-FDG PET imaging, and images acquired from two separate scans are superimposed for analysis. The 18F-FCH counts are subtracted from the merged images to identify the tumor. Micro-CT, bioluminescence imaging (BLI), histology and measurement of the tumor diameter are also conducted for comparison. Results show that there is a significant contrast in 18F-FCH uptake between tumor and normal brain tissue (2.65 ± 0.98), but with a high false positive rate of 28.6%. The difficulty of identifying the tumor by 18FFDG only is also proved in this study. All the tumors can be detected based on the dual tracer technique of 18F-FCH/ 18F-FDG-PET imaging in this study, while the false-positive caused by 18F-FCH can be eliminated. Dual tracer 18F-FCH/18F-FDG PET imaging has the potential to improve the visualization of low grade glioma. 18F-FCH delineates tumor areas and the tumor can be identified by subtracting the 18F-FCH counts. The sensitivity was over 95%. Further studies are required to evaluate the possibility of applying this technique in clinical trials. |
Issue Date: | 4-Feb-2016 |
Date of Acceptance: | 13-Jan-2016 |
URI: | http://hdl.handle.net/10044/1/69145 |
DOI: | https://doi.org/10.1371/journal.pone.0148123 |
ISSN: | 1932-6203 |
Publisher: | Public Library of Science (PLoS) |
Journal / Book Title: | PLoS ONE |
Volume: | 11 |
Issue: | 2 |
Copyright Statement: | © 2016 Fu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
Keywords: | Science & Technology Multidisciplinary Sciences Science & Technology - Other Topics POSITRON-EMISSION-TOMOGRAPHY MINIMALLY INVASIVE SURGERY RADIATION-DOSE ESCALATION C-11 CHOLINE PET GLIOBLASTOMA-MULTIFORME FOLLOW-UP F-18-FLUOROCHOLINE PROLIFERATION F-18-FDG GLIOMAS Animals Brain Neoplasms Cell Line, Tumor Choline Disease Models, Animal Fluorodeoxyglucose F18 Glioblastoma Heterografts Humans Image Processing, Computer-Assisted Luminescent Measurements Mice Positron-Emission Tomography Radiopharmaceuticals General Science & Technology MD Multidisciplinary |
Publication Status: | Published |
Article Number: | e0148123 |
Online Publication Date: | 2016-02-04 |
Appears in Collections: | Mechanical Engineering |