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Evaluation of the Luminex xTAG Respiratory Viral Panel FAST v2 assay for detection of multiple respiratory viral pathogens in nasal and throat swabs in Vietnam [version 1; referees: 2 approved]
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13cce207-4fed-4ffb-9c56-e867d7c9066b_12429_-_Ty_Hang_Vu.pdf | Published version | 843.81 kB | Adobe PDF | View/Open |
Title: | Evaluation of the Luminex xTAG Respiratory Viral Panel FAST v2 assay for detection of multiple respiratory viral pathogens in nasal and throat swabs in Vietnam [version 1; referees: 2 approved] |
Authors: | Thi Ty Hang, V Thi Han Ny, N My Phuc, T Thi Thanh Tam, P Thao Huong, D Dang Trung Nghia, H Tran Anh Vu, N Thi Hong Phuong, P Van Xang, N Dong, N Nhu Hiep, P Van Hung, N Tinh Hien, T Rabaa, M Thwaites, GE Baker, S Van Tan, L Van Doorn, HR Berto, A Boni, MF Bryant, JE Phu, BD Campbell, JI Carrique-Mas, J Hung, DM Huong, DT Oanh, DT Day, JN Tan, DV Han, DA Farrar, JJ Trang, HTT Long, HB Duong, HV Thu, HTK Cuong, LC Hung, LM Phuong, LT Phuc, LT Phuong, LT Luat, LX Ha, LTT Chuong, LV Loan, MTP Nadjm, B Bao, NT Hoa, NT Tue, NT Tu, NC Thuan, ND Chuyen, NK An, NN Vinh, NN Hung, NQ Dung, NT Minh, NT Binh, NT Tham, NTH Tien, NTH Chuc, NTK Ngoc, NTL Ha, NTL Lien, NTN Diep, NTN Nhung, NT Chau, NTS Chi, NTY Trinh, NT Van, NT Van Cuong, N Van Kinh, NV Van Minh Hoang, N Van My, N Van Thang, N Van Thanh, N Van Vinh Chau, N Ha My, P Hong Anh, P Thi Minh Khoa, P Van Lao, PV Van Minh, P Van Be Bay, P Rahman, M Thompson, C Thi Dieu Ngan, TTD Do Hoang Nhu, T Hoang Minh Chau, THM Toan, TK Thi Kim Hong, T Thi Ngoc Dung, T Thi Thanh Thanh, T Thi Thuy Minh, T Thua Nguyen, TT Tri, TQ Be Hien, V |
Item Type: | Journal Article |
Abstract: | Background: Acute respiratory infections (ARI) are among the leading causes of hospitalization in children ≤5 years old. Rapid diagnostics of viral pathogens is essential to avoid unnecessary antibiotic treatment, thereby slowing down antibiotic-resistance. We evaluated the diagnostic performance of the Luminex xTAG Respiratory Viral Panel FAST v2 against viral specific PCR as reference assays for ARI in Vietnam. Methods: Four hundred and forty two nose and throat swabs were collected in viral transport medium, and were tested with Luminex xTAG Respiratory Viral Panel FAST v2. Multiplex RT-PCR and single RT-PCR were used as references. Results: Overall, viral pathogens were detected in a total count of 270/294 (91.8%, 95% CI 88.1-94.7) by the Luminex among reference assays, whilst 112/6336 (1.8%, 95% CI, 1.4-2.1) of pathogens were detected by the Luminex, but not by reference assays. Frequency of pathogens detected by Luminex and reference assays was 379 and 292, respectively. The diagnostic yield was 66.7% (295/442, 95%CI 62.1-71.1%) for the Luminex assay and 54.1% (239/442, 95% CI, 49.3-58.8%) for reference assays. The Luminex kit had higher yields for all viruses except influenza B virus, respiratory syncytial virus, and human bocavirus. High agreements between both methods [mean (range): 0.91 (0.83-1.00)] were found for 10/15 viral agents. Conclusions: The Luminex assay is a high throughput multiplex platform for rapid detection of common viral pathogens causing ARI. Although the current high cost may prevent Luminex assays from being widely used, especially in limited resource settings where ARI are felt most, its introduction in clinical diagnostics may help reduce unnecessary use of antibiotic prescription. |
Issue Date: | 1-Sep-2017 |
Date of Acceptance: | 1-Sep-2017 |
URI: | http://hdl.handle.net/10044/1/67761 |
DOI: | https://dx.doi.org/10.12688/wellcomeopenres.12429.1 |
ISSN: | 2398-502X |
Publisher: | F1000Research |
Journal / Book Title: | Wellcome Open Research |
Volume: | 2 |
Copyright Statement: | © 2017 Thi Ty Hang V et al. This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Data associated with the article are available under the terms of the Creative Commons Zero "No rights reserved" data waiver (CC0 1.0 Public domain dedication). |
Publication Status: | Published |
Article Number: | ARTN 80 |
Online Publication Date: | 2017-09-05 |
Appears in Collections: | Department of Medicine (up to 2019) |