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Absence of nuclease activity in commonly used oxygen-scavenging systems
| File | Description | Size | Format | |
|---|---|---|---|---|
 2017 Gahlon BMR Research Notes.pdf | Published version | 1.24 MB | Adobe PDF | View/Open |
| Title: | Absence of nuclease activity in commonly used oxygen-scavenging systems |
| Authors: | Gahlon, HL Poudevigne-Durance, P Rueda, D |
| Item Type: | Journal Article |
| Abstract: | Objective Oxygen scavenging systems are routinely used during single-molecule imaging experiments to improve fluorescent dye stability. Previous work has shown nuclease contamination in the commonly used oxygen scavenging systems. This study evaluates the potential for nuclease contamination in these oxygen scavenging systems. Results Linear and plasmid DNA was incubated with two different oxygen scavenging systems (1) protocatechuic acid (PCA)-protocatechuate-3,4-dioxygenase (PCD) and (2) glucose-coupled glucose oxidase/catalase (GODCAT). No nucleic acid degradation was observed on single and double-stranded linear DNA and plasmid DNA, indicating the absence of nuclease contamination in these oxygen scavenging systems. |
| Issue Date: | 21-Nov-2017 |
| Date of Acceptance: | 14-Nov-2017 |
| URI: | http://hdl.handle.net/10044/1/54319 |
| DOI: | https://dx.doi.org/10.1186/s13104-017-2929-6 |
| ISSN: | 1756-0500 |
| Publisher: | BioMed Central |
| Journal / Book Title: | BMC Research Notes |
| Volume: | 10 |
| Copyright Statement: | © The Author(s) 2017. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/ ), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/ publicdomain/zero/1.0/ ) applies to the data made available in this article, unless otherwise stated. |
| Sponsor/Funder: | Commission of the European Communities Marie Skłodowska Curie Actions Medical Research Council |
| Funder's Grant Number: | 705015 705015 MC_UP_1102/5 |
| Keywords: | Catalase Glucose oxidase Oxygen scavenging system Protocatechuate-3,4-dioxygenase Protocatechuic acid Single-molecule imaging 1199 Other Medical And Health Sciences Bioinformatics |
| Publication Status: | Published |
| Article Number: | 606 |
| Appears in Collections: | Department of Medicine (up to 2019) |