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A 3D printed microfluidic device with integrated biosensors for online analysis of subcutaneous human microdialysate

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Final Manuscript file.pdfAccepted version2.82 MBAdobe PDFView/Open
Supporting Information.pdfSupporting information14.96 MBAdobe PDFView/Open
acs%2Eanalchem%2E5b01353.pdfPublished version2.52 MBAdobe PDFView/Open
Title: A 3D printed microfluidic device with integrated biosensors for online analysis of subcutaneous human microdialysate
Authors: Gowers, SAN
Curto, VF
Seneci, CA
Wang, C
Anastasova, S
Vadgama, P
Yang, G-Z
Boutelle, MG
Item Type: Journal Article
Abstract: This work presents the design, fabrication, and characterization of a robust 3D printed microfluidic analysis system that integrates with FDA-approved clinical microdialysis probes for continuous monitoring of human tissue metabolite levels. The microfluidic device incorporates removable needle type integrated biosensors for glucose and lactate, which are optimized for high tissue concentrations, housed in novel 3D printed electrode holders. A soft compressible 3D printed elastomer at the base of the holder ensures a good seal with the microfluidic chip. Optimization of the channel size significantly improves the response time of the sensor. As a proof-of-concept study, our microfluidic device was coupled to lab-built wireless potentiostats and used to monitor real-time subcutaneous glucose and lactate levels in cyclists undergoing a training regime.
Issue Date: 4-Aug-2015
Date of Acceptance: 12-Jun-2015
URI: http://hdl.handle.net/10044/1/23852
DOI: 10.1021/acs.analchem.5b01353
ISSN: 0003-2700
Publisher: American Chemical Society
Start Page: 7763
End Page: 7770
Journal / Book Title: Analytical Chemistry
Volume: 87
Issue: 15
Copyright Statement: © 2015 American Chemical Society. ACS AuthorChoice - This is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
Sponsor/Funder: Wellcome Trust
Engineering & Physical Science Research Council (EPSRC)
Engineering & Physical Science Research Council (EPSRC)
Funder's Grant Number: 094912/Z/10/Z
EP/H009744/1
EP/L014149/1
Keywords: Science & Technology
Physical Sciences
Chemistry, Analytical
Chemistry
RAPID SAMPLING MICRODIALYSIS
ELECTROANALYSIS
VERSATILE
NETWORKS
Biosensing Techniques
Electrodes
Glucose
Humans
Lactic Acid
Microdialysis
Microfluidic Analytical Techniques
Monitoring, Physiologic
Printing, Three-Dimensional
Humans
Lactic Acid
Glucose
Monitoring, Physiologic
Microdialysis
Microfluidic Analytical Techniques
Biosensing Techniques
Electrodes
Printing, Three-Dimensional
0301 Analytical Chemistry
0399 Other Chemical Sciences
Analytical Chemistry
Publication Status: Published
Online Publication Date: 2015-07-20
Appears in Collections:Bioengineering
Institute of Global Health Innovation
Faculty of Engineering



This item is licensed under a Creative Commons License Creative Commons