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A 3D printed microfluidic device with integrated biosensors for online analysis of subcutaneous human microdialysate
File | Description | Size | Format | |
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Final Manuscript file.pdf | Accepted version | 2.82 MB | Adobe PDF | View/Open |
Supporting Information.pdf | Supporting information | 14.96 MB | Adobe PDF | View/Open |
acs%2Eanalchem%2E5b01353.pdf | Published version | 2.52 MB | Adobe PDF | View/Open |
Title: | A 3D printed microfluidic device with integrated biosensors for online analysis of subcutaneous human microdialysate |
Authors: | Gowers, SAN Curto, VF Seneci, CA Wang, C Anastasova, S Vadgama, P Yang, G-Z Boutelle, MG |
Item Type: | Journal Article |
Abstract: | This work presents the design, fabrication, and characterization of a robust 3D printed microfluidic analysis system that integrates with FDA-approved clinical microdialysis probes for continuous monitoring of human tissue metabolite levels. The microfluidic device incorporates removable needle type integrated biosensors for glucose and lactate, which are optimized for high tissue concentrations, housed in novel 3D printed electrode holders. A soft compressible 3D printed elastomer at the base of the holder ensures a good seal with the microfluidic chip. Optimization of the channel size significantly improves the response time of the sensor. As a proof-of-concept study, our microfluidic device was coupled to lab-built wireless potentiostats and used to monitor real-time subcutaneous glucose and lactate levels in cyclists undergoing a training regime. |
Issue Date: | 4-Aug-2015 |
Date of Acceptance: | 12-Jun-2015 |
URI: | http://hdl.handle.net/10044/1/23852 |
DOI: | 10.1021/acs.analchem.5b01353 |
ISSN: | 0003-2700 |
Publisher: | American Chemical Society |
Start Page: | 7763 |
End Page: | 7770 |
Journal / Book Title: | Analytical Chemistry |
Volume: | 87 |
Issue: | 15 |
Copyright Statement: | © 2015 American Chemical Society. ACS AuthorChoice - This is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited. |
Sponsor/Funder: | Wellcome Trust Engineering & Physical Science Research Council (EPSRC) Engineering & Physical Science Research Council (EPSRC) |
Funder's Grant Number: | 094912/Z/10/Z EP/H009744/1 EP/L014149/1 |
Keywords: | Science & Technology Physical Sciences Chemistry, Analytical Chemistry RAPID SAMPLING MICRODIALYSIS ELECTROANALYSIS VERSATILE NETWORKS Biosensing Techniques Electrodes Glucose Humans Lactic Acid Microdialysis Microfluidic Analytical Techniques Monitoring, Physiologic Printing, Three-Dimensional Humans Lactic Acid Glucose Monitoring, Physiologic Microdialysis Microfluidic Analytical Techniques Biosensing Techniques Electrodes Printing, Three-Dimensional 0301 Analytical Chemistry 0399 Other Chemical Sciences Analytical Chemistry |
Publication Status: | Published |
Online Publication Date: | 2015-07-20 |
Appears in Collections: | Bioengineering Institute of Global Health Innovation Faculty of Engineering |
This item is licensed under a Creative Commons License