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A bacteriophage DNA mimic protein employs a non-specific strategy to inhibit the bacterial RNA polymerase

Title: A bacteriophage DNA mimic protein employs a non-specific strategy to inhibit the bacterial RNA polymerase
Authors: Wang, Z
Wang, H
Mulvenna, N
Sanz-Hernandez, M
Zhang, P
Li, Y
Ma, J
Wang, Y
Matthews, S
Wigneshweraraj, S
Liu, B
Item Type: Journal Article
Abstract: DNA mimicry by proteins is a strategy that employed by some proteins to occupy the binding sites of the DNA-binding proteins and deny further access to these sites by DNA. Such proteins have been found in bacteriophage, eukaryotic virus, prokaryotic, and eukaryotic cells to imitate non-coding functions of DNA. Here, we report another phage protein Gp44 from bacteriophage SPO1 of Bacillus subtilis, employing mimicry as part of unusual strategy to inhibit host RNA polymerase. Consisting of three simple domains, Gp44 contains a DNA binding motif, a flexible DNA mimic domain and a random-coiled domain. Gp44 is able to anchor to host genome and interact bacterial RNA polymerase via the β and β′ subunit, resulting in bacterial growth inhibition. Our findings represent a non-specific strategy that SPO1 phage uses to target different bacterial transcription machinery regardless of the structural variations of RNA polymerases. This feature may have potential applications like generation of genetic engineered phages with Gp44 gene incorporated used in phage therapy to target a range of bacterial hosts.
Issue Date: 2-Jun-2021
Date of Acceptance: 30-Apr-2021
URI: http://hdl.handle.net/10044/1/103068
DOI: 10.3389/fmicb.2021.692512
ISSN: 1664-302X
Publisher: Frontiers Media S.A.
Start Page: 1
End Page: 10
Journal / Book Title: Frontiers in Microbiology
Volume: 12
Copyright Statement: Copyright © 2021 Wang, Wang, Mulvenna, Sanz-Hernandez, Zhang, Li, Ma, Wang, Matthews, Wigneshweraraj and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Publication Status: Published
Article Number: ARTN 692512
Online Publication Date: 2021-06-02
Appears in Collections:Department of Infectious Diseases
Faculty of Natural Sciences



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