3
IRUS TotalDownloads
Altmetric
A bacteriophage DNA mimic protein employs a non-specific strategy to inhibit the bacterial RNA polymerase
File | Description | Size | Format | |
---|---|---|---|---|
A Bacteriophage DNA Mimic Protein Employs a Non-specific Strategy to Inhibit the Bacterial RNA Polymerase.pdf | Published version | 2.38 MB | Adobe PDF | View/Open |
Title: | A bacteriophage DNA mimic protein employs a non-specific strategy to inhibit the bacterial RNA polymerase |
Authors: | Wang, Z Wang, H Mulvenna, N Sanz-Hernandez, M Zhang, P Li, Y Ma, J Wang, Y Matthews, S Wigneshweraraj, S Liu, B |
Item Type: | Journal Article |
Abstract: | DNA mimicry by proteins is a strategy that employed by some proteins to occupy the binding sites of the DNA-binding proteins and deny further access to these sites by DNA. Such proteins have been found in bacteriophage, eukaryotic virus, prokaryotic, and eukaryotic cells to imitate non-coding functions of DNA. Here, we report another phage protein Gp44 from bacteriophage SPO1 of Bacillus subtilis, employing mimicry as part of unusual strategy to inhibit host RNA polymerase. Consisting of three simple domains, Gp44 contains a DNA binding motif, a flexible DNA mimic domain and a random-coiled domain. Gp44 is able to anchor to host genome and interact bacterial RNA polymerase via the β and β′ subunit, resulting in bacterial growth inhibition. Our findings represent a non-specific strategy that SPO1 phage uses to target different bacterial transcription machinery regardless of the structural variations of RNA polymerases. This feature may have potential applications like generation of genetic engineered phages with Gp44 gene incorporated used in phage therapy to target a range of bacterial hosts. |
Issue Date: | 2-Jun-2021 |
Date of Acceptance: | 30-Apr-2021 |
URI: | http://hdl.handle.net/10044/1/103068 |
DOI: | 10.3389/fmicb.2021.692512 |
ISSN: | 1664-302X |
Publisher: | Frontiers Media S.A. |
Start Page: | 1 |
End Page: | 10 |
Journal / Book Title: | Frontiers in Microbiology |
Volume: | 12 |
Copyright Statement: | Copyright © 2021 Wang, Wang, Mulvenna, Sanz-Hernandez, Zhang, Li, Ma, Wang, Matthews, Wigneshweraraj and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
Publication Status: | Published |
Article Number: | ARTN 692512 |
Online Publication Date: | 2021-06-02 |
Appears in Collections: | Department of Infectious Diseases Faculty of Natural Sciences |
This item is licensed under a Creative Commons License