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Body composition of the host influences dendritic cell phenotype in patients treated for colorectal cancer
File(s)
Author(s)
Malietzis, G
Lee, GH
Al-Hassi, HO
Bernardo, D
Blakemore, AI
Type
Journal Article
Abstract
Dendritic cells (DCs) are antigen-presenting cells that can acquire tumour antigens and initiate cytotoxic T cell reactions. Obesity has been proposed as a cause for tumours escaping immune surveillance, but few studies investigate the impact of other body composition parameters. We examined the relationship of DC phenotype with computer tomography (CT)-defined parameters in patients with colorectal cancer (CRC). DCs were identified within peripheral blood mononuclear cells by flow cytometry as HLA-DR positive and negative for markers of other cell lineages in 21 patients. Analysis of CT scans was used to calculate lumbar skeletal muscle index (LSMI) and mean muscle attenuation (MA). Positive correlation between the LSMI and expression of CD40 in all DCs (r = 0.45; p = 0.04) was demonstrated. The MA was positively correlated with scavenger receptor CD36 [all DCs (r = 0.60; p = 0.01) and myeloid DCs (r = 0.63; p < 0.01)]. However, the MA was negatively correlated with CCR7 expression in all DCs (r = −0.46, p = 0.03.) and with CD83 [all DCs (r = −0.63; p = 0.01) and myeloid DCs (r = −0.75; p < 0.01)]. There were no relationships between the fat indexes and the DC phenotype. These results highlight a direct relationship between muscle depletion and changes in stimulatory, migratory and fatty acid-processing potential of DC in patients with CRC.
Date Issued
2016-03-10
Date Acceptance
2016-03-01
Citation
Tumor Biology, 2016, 37 (8), pp.11359-11364
URI
DOI
ISSN
1423-0380
Publisher
Springer Verlag (Germany)
Start Page
11359
End Page
11364
Journal / Book Title
Tumor Biology
Volume
37
Issue
8
Copyright Statement
© Springer-Verlag 2016. The final publication is available at Springer via http:///dx.doi.org/10.1007/s13277-016-5009-y
Sponsor
Biotechnology and Biological Sciences Research Cou
Biotechnology and Biological Sciences Research Council
London North West Healthcare NHS Trust (LNWH)
Grant Number
PR5858-000-A
PR5858-000-A
n/a
Subjects
Body composition
Colorectal cancer
Dendritic cells
Myopenia
Oncology & Carcinogenesis
1103 Clinical Sciences
Publication Status
Published