Maternal fat intake during pregnancy in relation to offspring metabolic outcomes has been
studied primarily in animal models, yet little is known about the association in humans. The aim of this
study was to examine the association of total and subtype of fat consumption in pregnancy with
anthropometric measures and biomarkers of adiposity and glucose metabolism in the offspring.
Methods: A source population was 965 Danish pregnant women recruited in 1988e1989 with offspring
follow-up at 20 years. Information on fat intake was collected in the 30th week of gestation, and we
subdivided fat according to saturated (SFA), monounsaturated (MUFA), and polyunsaturated (PUFA) fat.
Offspring body mass index (BMI) and waist circumference (WC) were recorded at follow-up
(n ¼ 670678), and biomarkers were quantified in a subset (n ¼ 443) of participants. Multivariable
linear and log-binomial regression were used to calculate effect estimates and 95% CI for a 1:1%energy
substitution of carbohydrates for fat.
Results: The mean (standard deviation) BMI was 22.1 (3.3) and 22.8 (2.9) kg/m2 in female and male
offspring, respectively. The median (10th to 90th percentile) of maternal fat intake was 31% of energy
[23,39]. We found no overall associations for maternal fat intake with female offspring anthropometry.
However, for male offspring higher intake of MUFA during pregnancy was associated with higher insulin
levels at 20 years (Q4 vs. Q1: %D: 37, 95% CI: 1, 86) accompanied by a non-significant 3.6 (95% CI: 1.1,
8.2) cm increase in WC. High maternal total fat intake (>¼35% energy) was also associated with higher
BMI (0.9, 95% CI: 0.2, 1.6) and WC (4.0, 95% CI: 1.6, 2.3) among male offspring.
Conclusions: A high fat diet during pregnancy may increase adiposity in adult male offspring. We surmise
that maternal MUFA intake during this time included both MUFA and trans fat misclassified as MUFA, and
that the associations observed may be more reflective of the latter exposure.