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Quantitative and rapid Plasmodium falciparum malaria diagnosis and artemisinin-resistance detection using a CMOS Lab-on-Chip platform

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Title: Quantitative and rapid Plasmodium falciparum malaria diagnosis and artemisinin-resistance detection using a CMOS Lab-on-Chip platform
Authors: Malpartida-Cardenas, K
Miscourides, N
Rodriguez-Manzano, J
Yu, L-S
Moser, N
Baum, J
Georgiou, P
Item Type: Journal Article
Abstract: Early and accurate diagnosis of malaria and drug-resistance is essential to effective disease management. Available rapid malaria diagnostic tests present limitations in analytical sensitivity, drug-resistance testing and/or quantification. Conversely, diagnostic methods based on nucleic acid amplification stepped forwards owing to their high sensitivity, specificity and robustness. Nevertheless, these methods commonly rely on optical measurements and complex instrumentation which limit their applicability in resource-poor, point-of-care settings. This paper reports the specific, quantitative and fully-electronic detection of Plasmodium falciparum, the predominant malaria-causing parasite worldwide, using a Lab-on-Chip platform developed in-house. Furthermore, we demonstrate on-chip detection of C580Y, the most prevalent single-nucleotide polymorphism associated to artemisinin-resistant malaria. Real-time non-optical DNA sensing is facilitated using Ion-Sensitive Field-Effect Transistors, fabricated in unmodified complementary metal-oxide-semiconductor (CMOS) technology, coupled with loop-mediated isothermal amplification. This work holds significant potential for the development of a fully portable and quantitative malaria diagnostic that can be used as a rapid point-of-care test.
Issue Date: 1-Dec-2019
Date of Acceptance: 4-Sep-2019
URI: http://hdl.handle.net/10044/1/74069
DOI: https://dx.doi.org/10.1016/j.bios.2019.111678
ISSN: 0956-5663
Publisher: Elsevier BV
Journal / Book Title: Biosensors and Bioelectronics
Volume: 145
Copyright Statement: © 2019 Elsevier B.V. All rights reserved. This manuscript is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence http://creativecommons.org/licenses/by-nc-nd/4.0/
Sponsor/Funder: Wellcome Trust
Wellcome Trust
Engineering & Physical Science Research Council (E
Engineering & Physical Science Research Council (EPSRC)
Funder's Grant Number: 100993/Z/13/Z
100993/Z/13/Z
EP/P510798/1
EP/R512655/1
Keywords: CMOS
ISFET
LAMP
Lab-on-Chip
Malaria
P. falciparum
Point-of-Care
SNP
0301 Analytical Chemistry
0903 Biomedical Engineering
1007 Nanotechnology
Bioinformatics
Publication Status: Published
Embargo Date: 2020-09-07
Article Number: ARTN 111678
Online Publication Date: 2019-09-07
Appears in Collections:Electrical and Electronic Engineering
Faculty of Medicine
Faculty of Natural Sciences



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