Heteromeric RNP assembly at LINEs controls lineage-specific RNA processing

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Title: Heteromeric RNP assembly at LINEs controls lineage-specific RNA processing
Authors: Attig, J
Agostini, F
Gooding, C
Chakrabarti, AM
Singh, A
Haberman, N
Zagalak, JA
Emmett, W
Smith, CWJ
Luscombe, NM
Ule, J
Item Type: Journal Article
Abstract: Long mammalian introns make it challenging for the RNA processing machinery to identify exons accurately. We find that LINE-derived sequences (LINEs) contribute to this selection by recruiting dozens of RNA-binding proteins (RBPs) to introns. This includes MATR3, which promotes binding of PTBP1 to multivalent binding sites within LINEs. Both RBPs repress splicing and 3′ end processing within and around LINEs. Notably, repressive RBPs preferentially bind to evolutionarily young LINEs, which are located far from exons. These RBPs insulate the LINEs and the surrounding intronic regions from RNA processing. Upon evolutionary divergence, changes in RNA motifs within LINEs lead to gradual loss of their insulation. Hence, older LINEs are located closer to exons, are a common source of tissue-specific exons, and increasingly bind to RBPs that enhance RNA processing. Thus, LINEs are hubs for the assembly of repressive RBPs and also contribute to the evolution of new, lineage-specific transcripts in mammals.
Issue Date: 23-Aug-2018
Date of Acceptance: 1-Jul-2018
ISSN: 0092-8674
Publisher: Elsevier BV
Start Page: 1067
End Page: 1081.e17
Journal / Book Title: Cell
Volume: 174
Issue: 5
Copyright Statement: © 2018 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (
Keywords: Developmental Biology
06 Biological Sciences
11 Medical and Health Sciences
Publication Status: Published
Online Publication Date: 2018-08-02
Appears in Collections:Clinical Sciences
Molecular Sciences

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