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Chemically functionalised graphene FET biosensor for the label-free sensing of exosomes

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Title: Chemically functionalised graphene FET biosensor for the label-free sensing of exosomes
Authors: Kwong Hong Tsang, D
Lieberthal, T
Watts, C
Del Rio Hernandez, A
Dunlop, I
Ramadan, S
Klein, N
Item Type: Journal Article
Abstract: A graphene field-effect transistor (gFET) was non-covalently functionalised with 1-pyrenebutyric acid N-hydroxysuccinimide ester and conjugated with anti-CD63 antibodiesfor the label-free detection of exosomes.Using a microfluidic channel, part of a graphene film was exposed to solution. The change in electrical properties of the exposed graphene created anadditional minimum alongside the original Dirac point inthe drain-source current(Ids)-back-gate voltage (Vg) curve. When phosphate buffered saline (PBS) was present in the channel, the additional minimum was present at a Vglower than the original Dirac point and shifted with time when exosomes were introduced into the channel.Thisshift of the minimum from the PBS reference point reached saturationafter 30 minutesand was observed for multiple exosome concentrations. Upon conjugation with an isotype control, sensor responsetothe highest concentration ofexosomes was negligible in comparison to that with anti-CD63antibody, indicatingthat thefunctionalised gFETcan specifically detect exosomes at least down to 0.1μg/mLand is sensitive to concentration. Such a gFET biosensor has not been used before for exosome sensing and could be an effective tool for the liquid-biopsy detection of exosomes as biomarkers for early-stage identification of diseases such as cancer.
Issue Date: 26-Sep-2019
Date of Acceptance: 28-Aug-2019
URI: http://hdl.handle.net/10044/1/73270
DOI: https://dx.doi.org/10.1038/s41598-019-50412-9
ISSN: 2045-2322
Publisher: Nature Publishing Group
Journal / Book Title: Scientific Reports
Volume: 9
Copyright Statement: © 2019 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Sponsor/Funder: Engineering & Physical Science Research Council (EPSRC)
Engineering & Physical Science Research Council (EPSRC)
Funder's Grant Number: EP/P02985X/1
EP/M020398/1
Keywords: 0601 Biochemistry and Cell Biology
0299 Other Physical Sciences
Publication Status: Published
Article Number: ARTN 13946
Appears in Collections:Faculty of Engineering
Materials
Bioengineering
Faculty of Natural Sciences



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