Identification of putative miRNA biomarkers in early rheumatoid arthritis by genome-wide microarray profiling: A pilot study

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Title: Identification of putative miRNA biomarkers in early rheumatoid arthritis by genome-wide microarray profiling: A pilot study
Authors: Romo-García, MF
Bastian, Y
Zapata-Zuñiga, M
Macías-Segura, N
Castillo-Ortiz, JD
Lara-Ramírez, EE
Fernández-Ruíz, JC
Berlanga-Taylor, AJ
González-Amaro, R
Ramos-Remus, C
Enciso-Moreno, JA
Castañeda-Delgado, JE
Item Type: Journal Article
Abstract: Despite the existing research, the etiology of rheumatoid arthritis (RA), an autoimmune disease remains poorly understood with early and accurate diagnosis difficult to achieve. MicroRNAs (miRNAs) play an important role in biological processes as modulators of transcription and translation. Previous studies have demonstrated a downregulation of several genes in early RA stages and in addition, miRNAs may serve as early biomarkers of subclinical changes in early RA. When comparing the four groups (ANOVA P < 0.01, fold change > 4), we found 253 differentially expressed miRNAs. Of these, 97 miRNAs were identified as overexpressed in early rheumatoid arthritis. The validation of miRNA microarray expression was performed in a set by RT-qPCR and showed strong agreement with microarray expression data. The putative targets of overexpressed microRNAs in early RA were significantly enriched in apoptosis, tolerance loss and Wnt pathways. Moreover, ROC analysis showed values of AUC 0.76 and P < 0.05 for miR361-5p, identifying this miRNA as a potential biomarker of disease. We identified specific microRNAs associated with early rheumatoid arthritis and proposed them as early biomarkers of disease. Our results provide novel insight into immune disease physiopathology and describe unreported microRNAs in RA with potential for clinical use.
Issue Date: 15-Dec-2019
Date of Acceptance: 26-Aug-2019
URI: http://hdl.handle.net/10044/1/73068
DOI: https://doi.org/10.1016/j.gene.2019.144081
ISSN: 0378-1119
Publisher: Elsevier BV
Journal / Book Title: Gene
Volume: 720
Copyright Statement: © 2019 Elsevier Ltd. All rights reserved. This manuscript is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence http://creativecommons.org/licenses/by-nc-nd/4.0/
Sponsor/Funder: Medical Research Council (MRC)
Medical Research Council (MRC)
Medical Research Council
Funder's Grant Number: MR/L01632X/1
MR/L01632X/1
MEDBIO UK
Keywords: Biomarker
CCP
Early rheumatoid arthritis
Microarray expression
miRNA
Adult
Arthritis, Rheumatoid
Biomarkers
Case-Control Studies
Female
Gene Expression Profiling
Genome, Human
Humans
Male
MicroRNAs
Middle Aged
Pilot Projects
ROC Curve
Developmental Biology
0604 Genetics
Publication Status: Published online
Embargo Date: 2020-08-29
Article Number: 144081
Online Publication Date: 2019-08-29
Appears in Collections:Faculty of Medicine
Epidemiology, Public Health and Primary Care



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