The RNA binding protein Larp1 regulates cell division, apoptosis and cell migration

Title: The RNA binding protein Larp1 regulates cell division, apoptosis and cell migration
Authors: Burrows, C
Latip, NA
Lam, S-J
Carpenter, L
Sawicka, K
Tzolovsky, G
Gabra, H
Bushell, M
Glover, DM
Willis, AE
Blagden, SP
Item Type: Journal Article
Abstract: The RNA binding protein Larp1 was originally shown to be involved in spermatogenesis, embryogenesis and cell-cycle progression in Drosophila. Our data show that mammalian Larp1 is found in a complex with poly A binding protein and eukaryote initiation factor 4E and is associated with 60S and 80S ribosomal subunits. A reduction in Larp1 expression by siRNA inhibits global protein synthesis rates and results in mitotic arrest and delayed cell migration. Consistent with these data we show that Larp1 protein is present at the leading edge of migrating cells and interacts directly with cytoskeletal components. Taken together, these data suggest a role for Larp1 in facilitating the synthesis of proteins required for cellular remodelling and migration.
Issue Date: 1-Sep-2010
Date of Acceptance: 7-Apr-2010
URI: http://hdl.handle.net/10044/1/71745
DOI: https://doi.org/10.1093/nar/gkq294
ISSN: 0305-1048
Publisher: Oxford University Press
Start Page: 5542
End Page: 5553
Journal / Book Title: Nucleic Acids Research
Volume: 38
Issue: 16
Copyright Statement: © 2010 The Author(s). Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/ by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
POLY(A)-BINDING PROTEIN
TRANSLATION INITIATION
MECHANISMS
DROSOPHILA
FILOPODIA
COMPLEX
ORTHOLOGUE
DYNAMICS
MOTILITY
INVASION
Actins
Apoptosis
Autoantigens
Cell Movement
Cytoskeletal Proteins
Eukaryotic Initiation Factor-4E
HeLa Cells
Humans
Mitosis
Peptide Chain Initiation, Translational
Poly(A)-Binding Proteins
Pseudopodia
Ribonucleoproteins
Hela Cells
Pseudopodia
Humans
Actins
Poly(A)-Binding Proteins
Ribonucleoproteins
Eukaryotic Initiation Factor-4E
Cytoskeletal Proteins
Autoantigens
Mitosis
Apoptosis
Cell Movement
Peptide Chain Initiation, Translational
Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
POLY(A)-BINDING PROTEIN
TRANSLATION INITIATION
MECHANISMS
DROSOPHILA
FILOPODIA
COMPLEX
ORTHOLOGUE
DYNAMICS
MOTILITY
INVASION
05 Environmental Sciences
06 Biological Sciences
08 Information and Computing Sciences
Developmental Biology
Publication Status: Published
Online Publication Date: 2010-04-29
Appears in Collections:Division of Surgery
Division of Cancer



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