Spot and cumulative urine samples are suitable replacements for 24-hour urine collections for objective measures of dietary exposure in adults using metabolite biomarkers

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Title: Spot and cumulative urine samples are suitable replacements for 24-hour urine collections for objective measures of dietary exposure in adults using metabolite biomarkers
Authors: Wilson, T
Garcia-Perez, I
Posma, JM
Lloyd, AJ
Chambers, ES
Tailliart, K
Zubair, H
Beckmann, M
Mathers, JC
Holmes, E
Frost, G
Draper, J
Item Type: Journal Article
Abstract: BACKGROUND: Measurement of multiple food intake exposure biomarkers in urine may offer an objective method for monitoring diet. The potential of spot and cumulative urine samples that have reduced burden on participants as replacements for 24-h urine collections has not been evaluated. OBJECTIVE: The aim of this study was to determine the utility of spot and cumulative urine samples for classifying the metabolic profiles of people according to dietary intake when compared with 24-h urine collections in a controlled dietary intervention study. METHODS: Nineteen healthy individuals (10 male, 9 female, aged 21-65 y, BMI 20-35 kg/m2) each consumed 4 distinctly different diets, each for 1 wk. Spot urine samples were collected ∼2 h post meals on 3 intervention days/wk. Cumulative urine samples were collected daily over 3 separate temporal periods. A 24-h urine collection was created by combining the 3 cumulative urine samples. Urine samples were analyzed with metabolite fingerprinting by both high-resolution flow infusion electrospray mass spectrometry (FIE-HRMS) and proton nuclear magnetic resonance spectroscopy (1H-NMR). Concentrations of dietary intake biomarkers were measured with liquid chromatography triple quadrupole mass spectrometry and by integration of 1H-NMR data. RESULTS: Cross-validation modeling with 1H-NMR and FIE-HRMS data demonstrated the power of spot and cumulative urine samples in predicting dietary patterns in 24-h urine collections. Particularly, there was no significant loss of information when post-dinner (PD) spot or overnight cumulative samples were substituted for 24-h urine collections (classification accuracies of 0.891 and 0.938, respectively). Quantitative analysis of urine samples also demonstrated the relation between PD spot samples and 24-h urines for dietary exposure biomarkers. CONCLUSIONS: We conclude that PD spot urine samples are suitable replacements for 24-h urine collections. Alternatively, cumulative samples collected overnight predict similarly to 24-h urine samples and have a lower collection burden for participants.
Issue Date: 26-Jun-2019
Date of Acceptance: 24-May-2019
URI: http://hdl.handle.net/10044/1/71028
DOI: https://doi.org/10.1093/jn/nxz138
ISSN: 0022-3166
Publisher: Oxford University Press
Journal / Book Title: Journal of Nutrition
Copyright Statement: © 2019 Oxford University Press. This is a pre-copy-editing, author-produced version of an article accepted for publication in Journal of Nutrition following peer review. The definitive publisher-authenticated version Thomas Wilson, Isabel Garcia-Perez, Joram M Posma, Amanda J Lloyd, Edward S Chambers, Kathleen Tailliart, Hassan Zubair, Manfred Beckmann, John C Mathers, Elaine Holmes, Gary Frost, John Draper, Spot and Cumulative Urine Samples Are Suitable Replacements for 24-Hour Urine Collections for Objective Measures of Dietary Exposure in Adults Using Metabolite Biomarkers, The Journal of Nutrition, , nxz138, https://doi.org/10.1093/jn/nxz138 is available online at: https://doi.org/10.1093/jn/nxz138.
Sponsor/Funder: National Institute for Health Research
Funder's Grant Number: NIHR-PDF-2012-05-456
Keywords: 24-h urine
dietary intake
high-resolution mass spectrometry
metabolomics
nuclear magnetic resonance spectroscopy
spot urine
24-h urine
dietary intake
high-resolution mass spectrometry
metabolomics
nuclear magnetic resonance spectroscopy
spot urine
Nutrition & Dietetics
1111 Nutrition and Dietetics
0702 Animal Production
0908 Food Sciences
Publication Status: Published online
Conference Place: United States
Embargo Date: 2020-06-26
Online Publication Date: 2019-06-26
Appears in Collections:Division of Surgery
Department of Medicine



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