Germline selection shapes human mitochondrial DNA diversity

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Title: Germline selection shapes human mitochondrial DNA diversity
Authors: Wei, W
Tuna, S
Keogh, MJ
Smith, KR
Aitman, TJ
Beales, PL
Bennett, DL
Gale, DP
Bitner-Glindzicz, MAK
Black, GC
Brennan, P
Elliott, P
Flinter, FA
Floto, RA
Houlden, H
Irving, M
Koziell, A
Maher, ER
Markus, HS
Morrell, NW
Newman, WG
Roberts, I
Sayer, JA
Smith, KGC
Taylor, JC
Watkins, H
Webster, AR
Wilkie, AOM
Williamson, C
Attwood, A
Brown, M
Brod, NC
Crisp-Hihn, A
Davis, J
Deevi, SVV
Dewhurst, EF
Edwards, K
Erwood, M
Fox, J
Frary, AJ
Hu, F
Jolley, J
Kingston, N
Linger, R
Mapeta, R
Martin, J
Meacham, S
Papadia, S
Rayner-Matthews, PJ
Samarghitean, C
Shamardina, O
Simeoni, I
Staines, S
Staples, E
Stark, H
Stephens, J
Titterton, C
Tuna, S
Von Ziegenweidt, J
Watt, C
Whitehorn, D
Wood, Y
Yates, K
Yu, P
James, R
Ashford, S
Penkett, CJ
Stirrups, KE
Bariana, T
Lentaigne, C
Sivapalaratnam, S
Westbury, SK
Allsup, DJ
Bakchoul, T
Biss, T
Boyce, S
Collins, J
Collins, PW
Curry, NS
Downes, K
Dutt, T
Erber, WN
Evans, G
Everington, T
Favier, R
Gomez, K
Greene, D
Gresele, P
Hart, D
Kazmi, R
Kelly, AM
Lambert, M
Madan, B
Mangles, S
Mathias, M
Millar, C
Obaji, S
Peerlinck, K
Roughley, C
Schulman, S
Scully, M
Shapiro, SE
Sibson, K
Simeoni, I
Sims, MC
Tait, RC
Talks, K
Thys, C
Toh, C-H
Van Geet, C
Westwood, J-P
Papadia, S
Mumford, AD
Ouwehand, WH
Freson, K
Laffan, MA
Tan, RYY
Harkness, K
Mehta, S
Muir, KW
Hassan, A
Traylor, M
Drazyk, AM
Markus, HS
Parry, D
Ahmed, M
Kazkaz, H
Vandersteen, AM
Aitman, TJ
Ormondroyd, E
Thomson, K
Dent, T
Brennan, P
Buchan, RJ
Bueser, T
Carr-White, G
Cook, S
Daniels, MJ
Harper, AR
Ware, JS
Watkins, H
Dixon, PH
Chambers, J
Cheng, F
Estiu, MC
Hague, WM
Marschall, H-U
Vazquez-Lopez, M
Williamson, C
Arno, G
Dewhurst, EF
Erwood, M
French, CE
Michaelides, M
Moore, AT
Sanchis-Juan, A
Carss, K
Webster, AR
Raymond, FL
Chinnery, PF
Griffiths, P
Horvath, R
Hudson, G
Jurkute, N
Pyle, A
Wei, W
Yu-Wai-Man, P
Whitworth, J
Adlard, J
Ahmed, M
Armstrong, R
Brewer, C
Casey, R
Cole, TRP
Evans, DG
Greenhalgh, L
Hanson, HL
Hoffman, J
Izatt, L
Kumar, A
Lalloo, F
Ong, KR
Park, S-M
Searle, C
Side, L
Snape, K
Woodward, E
Tischkowitz, M
Maher, ER
Carss, K
Dewhurst, EF
Erwood, M
French, CE
Grozeva, D
Kurian, MA
Themistocleous, AC
Gosal, D
Horvath, R
Marshall, A
Matthews, E
McCarthy, MI
Renton, T
Rice, ASC
Vale, T
Walker, SM
Woods, CG
Bennett, DL
Thaventhiran, JE
Allen, HL
Savic, S
Alachkar, H
Antrobus, R
Baxendale, HE
Browning, MJ
Buckland, MS
Cooper, N
Edgar, JDM
Egner, W
Gilmour, KC
Goddard, S
Gordins, P
Grigoriadou, S
Hackett, S
Hague, R
Hayman, G
Herwadkar, A
Huissoon, AP
Jolles, S
Kelleher, P
Kumararatne, D
Longhurst, H
Lorenzo, LE
Lyons, PA
Maimaris, J
Noorani, S
Richter, A
Samarghitean, C
Sargur, RB
Sewell, WAC
Simeoni, I
Staples, E
Thomas, D
Thomas, MJ
Worth, A
Yong, PFK
Kuijpers, TW
Thrasher, AJ
Smith, KGC
Levine, AP
Sadeghi-Alavijeh, O
Wong, EKS
Cook, HT
Chan, MMY
Hall, M
Harris, C
McAlinden, P
Marchbank, KJ
Marks, S
Maxwell, H
Mozere, M
Wessels, J
Johnson, SA
Gale, DP
Bleda, M
Hadinnapola, C
Haimel, M
Swietlik, E
Bogaard, H
Church, C
Coghlan, G
Condliffe, R
Corris, P
Danesino, C
Eyries, M
Gall, H
Ghofrani, H-A
Gibbs, JSR
Girerd, B
Holden, S
Houweling, A
Howard, LS
Humbert, M
Kiely, DG
Kovacs, G
Lawrie, A
Ross, RVM
Martin, J
Moledina, S
Montani, D
Newnham, M
Olschewski, A
Olschewski, H
Peacock, A
Pepke-Zaba, J
Scelsi, L
Seeger, W
Soubrier, F
Suntharalingam, J
Toshner, M
Treacy, C
Trembath, R
Noordegraaf, AV
Waisfisz, Q
Wharton, J
Wilkins, MR
Wort, SJ
Yates, K
Graf, S
Morrell, NW
Louka, E
Roy, NB
Rao, A
Ancliff, P
Babbs, C
Layton, DM
Mead, AJ
O'Sullivan, J
Okoli, S
Roberts, I
Saleem, M
Bierzynska, A
Diz, CB
Colby, E
Ekani, MN
Satchell, S
Koziell, A
Fowler, T
Rendon, A
Scott, R
Smedley, D
Smith, KR
Thomas, E
Caulfield, M
Abbs, S
Burrows, N
Chitre, M
Dewhurst, EF
Floto, RA
Gattens, M
Gurnell, M
Holden, S
Kelsall, W
Mehta, S
Poole, KES
Ross-Russell, R
Spasic-Boskovic, O
Twiss, P
Wagner, A
Banka, S
Black, GC
Clayton-Smith, J
Douzgou, S
Newman, WG
Abulhoul, L
Aurora, P
Bockenhauer, D
Cleary, M
Dattani, M
Ganesan, V
Pilkington, C
Rahman, S
Shah, N
Wedderburn, L
Bitner-Glindzicz, MAK
Bueser, T
Compton, CJ
Deshpande, C
Fassihi, H
Haque, E
Izatt, L
Josifova, D
Mohammed, SN
Robert, L
Rose, SJ
Ruddy, DM
Sarkany, RN
Sayer, G
Shaw, AC
Irving, M
Flinter, FA
Campbell, C
Gibson, K
Koelling, N
Lester, T
Nemeth, AH
Palles, C
Patel, S
Roy, NB
Sen, A
Taylor, J
Tomlinson, IP
Taylor, JC
Wilkie, AOM
Arno, G
Malka, S
Michaelides, M
Moore, AT
Webster, AR
Brennan, P
Browning, AC
Burn, J
Chinnery, PF
De Soyza, A
Graham, J
Horvath, R
Pearce, S
Quinton, R
Schaefer, AM
Wilson, BT
Wright, M
Yu-Wai-Man, P
Sayer, JA
Simpson, M
Syrris, P
Elliott, P
Houlden, H
Beales, PL
Ashford, S
Penkett, CJ
Stirrups, KE
Rendon, A
Ouwehand, WH
Bradley, JR
Raymond, FL
Caulfield, M
Turro, E
Chinnery, PF
Item Type: Journal Article
Abstract: INTRODUCTION Only 2.4% of the 16.5-kb mitochondrial DNA (mtDNA) genome shows homoplasmic variation at >1% frequency in humans. Migration patterns have contributed to geographic differences in the frequency of common genetic variants, but population genetic evidence indicates that selection shapes the evolving mtDNA phylogeny. The mechanism and timing of this process are not clear. Unlike the nuclear genome, mtDNA is maternally transmitted and there are many copies in each cell. Initially, a new genetic variant affects only a proportion of the mtDNA (heteroplasmy). During female germ cell development, a reduction in the amount of mtDNA per cell causes a “genetic bottleneck,” which leads to rapid segregation of mtDNA molecules and different levels of heteroplasmy between siblings. Although heteroplasmy is primarily governed by random genetic drift, there is evidence of selection occurring during this process in animals. Yet it has been difficult to demonstrate this convincingly in humans. RATIONALE To determine whether there is selection for or against heteroplasmic mtDNA variants during transmission, we studied 12,975 whole-genome sequences, including 1526 mother–offspring pairs of which 45.1% had heteroplasmy affecting >1% of mtDNA molecules. Harnessing both the mtDNA and nuclear genome sequences, we then determined whether the nuclear genetic background influenced mtDNA heteroplasmy, validating our findings in another 40,325 individuals. RESULTS Previously unknown mtDNA variants were less likely to be inherited than known variants, in which the level of heteroplasmy tended to increase on transmission. Variants in the ribosomal RNA genes were less likely to be transmitted, whereas variants in the noncoding displacement (D)–loop were more likely to be transmitted. MtDNA variants predicted to affect the protein sequence tended to have lower heteroplasmy levels than synonymous variants. In 12,975 individuals, we identified a correlation between the location of heteroplasmic sites and known D-loop polymorphisms, including the absence of variants in critical sites required for mtDNA transcription and replication. We defined 206 unrelated individuals for which the nuclear and mitochondrial genomes were from different human populations. In these individuals, new population-specific heteroplasmies were more likely to match the nuclear genetic ancestry than the mitochondrial genome on which the mutations occurred. These findings were independently replicated in 654 additional unrelated individuals. CONCLUSION The characteristics of mtDNA in the human population are shaped by selective forces acting on heteroplasmy within the female germ line and are influenced by the nuclear genetic background. The signature of selection can be seen over one generation, ensuring consistency between these two independent genetic systems.
Issue Date: 24-May-2019
Date of Acceptance: 3-Apr-2019
ISSN: 0036-8075
Publisher: American Association for the Advancement of Science
Journal / Book Title: Science
Volume: 364
Issue: 6442
Copyright Statement: © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works This is an article distributed under the terms of the Science Journals Default License. This is the author’s version of the work. It is posted here by permission of the AAAS for personal use, not for redistribution. The definitive version was published in Science Vol 364 on 24 May 2019, DOI: 10.1126/science.aau6520
Sponsor/Funder: Medical Research Council (MRC)
Wellcome Trust
Funder's Grant Number: MR/J011711/1
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
NIHR BioResource–Rare Diseases
100,000 Genomes Project–Rare Diseases Pilot
Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
General Science & Technology
MD Multidisciplinary
Publication Status: Published
Article Number: eaau6520
Appears in Collections:National Heart and Lung Institute
Department of Medicine

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