Acrolein depletes the neuropeptides CGRP and substance P in sensory nerves in rat respiratory tract

Title: Acrolein depletes the neuropeptides CGRP and substance P in sensory nerves in rat respiratory tract
Authors: SPRINGALL, DR
EDGINTON, JAG
PRICE, PN
SWANSTON, DW
NOEL, C
BLOOM, SR
POLAK, JM
Item Type: Journal Article
Abstract: The mammalian respiratory tract is densely innervated by autonomic and sensory nerves around airways and blood vessels. Subsets of these nerves contain a number of putative neurotransmitter peptides, such as substance P and calcitonin gene-related peptide (CGRP) in sensory nerves and vasoactive intestinal polypeptide (VIP), possibly serving autonomic functions. CGRP is also found in endocrine cells in rat airway epithelium. These peptides are all pharmacologically potent effectors of bronchial and vascular smooth muscle and bronchial secretion. Their functions in vivo are less well established. We have therefore examined the effects of inhaled acrolein, a sensory irritant, on three pulmonary neuropeptides: CGRP, substance P, and VIP. Groups of rats (n = 3 each) were exposed for 10 min to acrolein in air (Ct = 510, 1858, and $5693\ {\rm mg}\bullet {\rm min}/{\rm m}^{3}$) or to air alone. Fifteen minutes later they were killed (pentabarbitone IP) and their respiratory tracts were dissected and fixed in 0.4% p-benzoquinone solution. Cryostat sections were stained by indirect immunofluorescence for a general nerve marker (PGP 9.5) and neuropeptides. The acrolein-treated animals had a dose-related decrease in tracheal substance P- and CGRP-immunoreactive nerve fibers compared with controls. No change was seen in total nerve fiber distribution and number (PGP 9.5) or VIP immunoreactivity, nor in CGRP-immunoreactive epithelial endocrine cells. It is concluded that the rat tracheal peptidergic nerves are a sensitive indicator of inhaled irritant substances. Their reduced immunoreactivity may be because of a release of sensory neuropeptides that could play a role in the physiological response to irritant or toxic compounds.
Issue Date: 1-Apr-1990
Date of Acceptance: 1-Apr-1990
URI: http://hdl.handle.net/10044/1/70679
DOI: https://doi.org/10.2307/3430678
ISSN: 0091-6765
Publisher: The National Institute of Environmental Health Sciences (NIEHS)
Start Page: 151
End Page: 157
Journal / Book Title: Environmental Health Perspectives
Volume: 85
Copyright Statement: © 1990 Environmental Health Perspectives.
Keywords: Science & Technology
Life Sciences & Biomedicine
Environmental Sciences
Public, Environmental & Occupational Health
Toxicology
Environmental Sciences & Ecology
Acrolein
Administration, Inhalation
Aldehydes
Animals
Calcitonin Gene-Related Peptide
Dose-Response Relationship, Drug
Female
Lung
Nerve Fibers
Neuropeptides
Rats
Substance P
Trachea
Ubiquitin Thiolesterase
Vasoactive Intestinal Peptide
Lung
Trachea
Nerve Fibers
Animals
Rats
Aldehydes
Acrolein
Vasoactive Intestinal Peptide
Ubiquitin Thiolesterase
Substance P
Neuropeptides
Calcitonin Gene-Related Peptide
Administration, Inhalation
Dose-Response Relationship, Drug
Female
Science & Technology
Life Sciences & Biomedicine
Environmental Sciences
Public, Environmental & Occupational Health
Toxicology
Environmental Sciences & Ecology
11 Medical and Health Sciences
05 Environmental Sciences
Toxicology
Publication Status: Published
Online Publication Date: 1990-04-01
Appears in Collections:Department of Medicine



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Creative Commons