A sensitised RNAi screen reveals a ch-TOG genetic interaction network required for spindle assembly

Title: A sensitised RNAi screen reveals a ch-TOG genetic interaction network required for spindle assembly
Authors: Barr, AR
Bakal, C
Item Type: Journal Article
Abstract: How multiple spindle assembly pathways are integrated to drive bipolar spindle assembly is poorly understood. We performed an image-based double RNAi screen to identify genes encoding Microtubule-Associated Proteins (MAPs) that interact with the highly conserved ch-TOG gene to regulate bipolar spindle assembly in human cells. We identified a ch-TOG centred network of genetic interactions which promotes centrosome-mediated microtubule polymerisation, leading to the incorporation of microtubules polymerised by all pathways into a bipolar structure. Our genetic screen also reveals that ch-TOG maintains a dynamic microtubule population, in part, through modulating HSET activity. ch-TOG ensures that spindle assembly is robust to perturbation but sufficiently dynamic such that spindles can explore a diverse shape space in search of structures that can align chromosomes.
Issue Date: 3-Jun-2015
Date of Acceptance: 20-Apr-2015
URI: http://hdl.handle.net/10044/1/70558
DOI: https://doi.org/10.1038/srep10564
ISSN: 2045-2322
Publisher: Nature Publishing Group
Journal / Book Title: Scientific Reports
Volume: 5
Copyright Statement: © 2015 Authors. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
XENOPUS EGG EXTRACTS
MICROTUBULE-ASSOCIATED PROTEIN
CROSS-LINKS MICROTUBULES
SISTER-CHROMATID COHESION
MITOTIC-SPINDLE
PLUS-END
INTERACTION MAP
CELL-DIVISION
CHROMOSOME CONGRESSION
SIGNALING NETWORKS
Chromatin
Cluster Analysis
Dyneins
Epistasis, Genetic
Gene Expression Profiling
Gene Regulatory Networks
Humans
Kinesin
Microtubule-Associated Proteins
Microtubules
Mitosis
Protein Binding
RNA Interference
RNA, Small Interfering
Spindle Apparatus
Tubulin
Chromatin
Microtubules
Humans
Tubulin
Kinesin
Microtubule-Associated Proteins
RNA, Small Interfering
Cluster Analysis
Gene Expression Profiling
Mitosis
Epistasis, Genetic
RNA Interference
Protein Binding
Gene Regulatory Networks
Dyneins
Spindle Apparatus
Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
XENOPUS EGG EXTRACTS
MICROTUBULE-ASSOCIATED PROTEIN
CROSS-LINKS MICROTUBULES
SISTER-CHROMATID COHESION
MITOTIC-SPINDLE
PLUS-END
INTERACTION MAP
CELL-DIVISION
CHROMOSOME CONGRESSION
SIGNALING NETWORKS
Publication Status: Published
Article Number: 10564
Online Publication Date: 2015-06-03
Appears in Collections:Clinical Sciences
Molecular Sciences



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