Defined conditions for propagation and manipulation of mouse embryonic stem cells

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Title: Defined conditions for propagation and manipulation of mouse embryonic stem cells
Authors: Mulas, C
Kalkan, T
Von Meyenn, F
Leitch, HG
Nichols, J
Smith, A
Item Type: Journal Article
Abstract: The power of mouse embryonic stem (ES) cells to colonise the developing embryo has revolutionised mammalian developmental genetics and stem cell research. This power is vulnerable, however, to the cell culture environment, deficiencies in which can lead to cellular heterogeneity, adaptive phenotypes, epigenetic aberrations and genetic abnormalities. Here, we provide detailed methodologies for derivation, propagation, genetic modification and primary differentiation of ES cells in 2i or 2i+LIF media without serum or undefined serum substitutes. Implemented diligently, these procedures minimise variability and deviation, thereby improving the efficiency, reproducibility and biological validity of ES cell experimentation.
Issue Date: 1-Mar-2019
Date of Acceptance: 19-Feb-2019
URI: http://hdl.handle.net/10044/1/70549
DOI: https://doi.org/10.1242/dev.173146
ISSN: 0950-1991
Publisher: Company of Biologists
Journal / Book Title: Delepment
Volume: 146
Issue: 6
Copyright Statement: © 2019. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
Keywords: Science & Technology
Life Sciences & Biomedicine
Developmental Biology
Differentiation
Embryonic stem cells
Pluripotency
Self-renewal
GROUND-STATE
NAIVE PLURIPOTENCY
SELF-RENEWAL
GERM-CELLS
IN-VITRO
GENOME
DIFFERENTIATION
DEMETHYLATION
DERIVATION
INHIBITION
Differentiation
Embryonic stem cells
Pluripotency
Self-renewal
Science & Technology
Life Sciences & Biomedicine
Developmental Biology
Differentiation
Embryonic stem cells
Pluripotency
Self-renewal
GROUND-STATE
NAIVE PLURIPOTENCY
SELF-RENEWAL
GERM-CELLS
IN-VITRO
GENOME
DIFFERENTIATION
DEMETHYLATION
DERIVATION
INHIBITION
06 Biological Sciences
11 Medical and Health Sciences
Publication Status: Published
Open Access location: http://dev.biologists.org/content/146/6/dev173146
Article Number: dev173146
Online Publication Date: 2019-03-26
Appears in Collections:Clinical Sciences
Imaging Sciences



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