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Effects of vancomycin and ciprofloxacin on the NMRI mouse metabolism

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Title: Effects of vancomycin and ciprofloxacin on the NMRI mouse metabolism
Authors: Liu, Z
Xia, B
Saric, J
Utzinger, J
Holmes, E
Keiser, J
Li, JV
Item Type: Journal Article
Abstract: The reduction in gut microbiota diversity is associated with a range of human diseases. Overuse of antibiotics has been associated with a diminished gut-microbial diversity in humans and may promote microbiota-associated negative effects to physical health, such as the metabolic syndrome-cluster of diseases and mental illnesses. There is a pressing need to deepen the understanding of the effects of antibiotics at the biochemical level. The current study investigated metabolic effects of two widely prescribed antibiotics–vancomycin and ciprofloxacin–on biofluids and brain tissue samples of NMRI female mice using a 1H nuclear magnetic resonance (NMR) spectroscopy-based metabolic profiling approach. While both antibiotics significantly affected the host metabolic signatures of urine and feces, only ciprofloxacin induced metabolic changes in plasma. Metabolic perturbations were pronounced 1 day post-treatment, reverting back to baseline at day 20 post-treatment. Both antibiotics induced changes in the choline metabolism, host-microbial cometabolites, short chain fatty acid production, and protein/purine degradation. The metabolic profiles of brain tissue aqueous extracts did not show any antibiotics-related changes by day 20 post-treatment. The data suggest that the metabolic disruptions in biofluids caused by antibiotics are reversed by day 20 post-treatment when compared to the pre-treatment profiles.
Issue Date: 5-Sep-2018
Date of Acceptance: 1-Sep-2018
URI: http://hdl.handle.net/10044/1/70402
DOI: https://dx.doi.org/10.1021/acs.jproteome.8b00583
ISSN: 1535-3893
Publisher: American Chemical Society
Start Page: 3565
End Page: 3573
Journal / Book Title: Journal of Proteome Research
Volume: 17
Issue: 10
Copyright Statement: © 2018 American Chemical Society
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemical Research Methods
Biochemistry & Molecular Biology
antibiotics
ciprofloxacin
vancomycin
H-1 nuclear magnetic resonance (NMR) spectroscopy
metabolic profiling
multivariate statistical analysis
MAGNETIC-RESONANCE-SPECTROSCOPY
AROMATIC CATABOLIC PATHWAYS
TRIMETHYLAMINE-N-OXIDE
GUT MICROBIOTA
ACID
DEGRADATION
HEALTH
FERMENTATION
MICROFLORA
ENZYMES
1H nuclear magnetic resonance (NMR) spectroscopy
antibiotics
ciprofloxacin
metabolic profiling
multivariate statistical analysis
vancomycin
Science & Technology
Life Sciences & Biomedicine
Biochemical Research Methods
Biochemistry & Molecular Biology
antibiotics
ciprofloxacin
vancomycin
H-1 nuclear magnetic resonance (NMR) spectroscopy
metabolic profiling
multivariate statistical analysis
MAGNETIC-RESONANCE-SPECTROSCOPY
AROMATIC CATABOLIC PATHWAYS
TRIMETHYLAMINE-N-OXIDE
GUT MICROBIOTA
ACID
DEGRADATION
HEALTH
FERMENTATION
MICROFLORA
ENZYMES
06 Biological Sciences
03 Chemical Sciences
Biochemistry & Molecular Biology
Publication Status: Published
Online Publication Date: 2018-09-05
Appears in Collections:Division of Surgery
Faculty of Medicine



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