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UbasM: An effective balanced optical clearing method for intact biomedical imaging

Title: UbasM: An effective balanced optical clearing method for intact biomedical imaging
Authors: Chen, L
Li, G
Li, Y
Li, Y
Zhu, H
Tang, L
French, P
McGinty, J
Ruan, S
Item Type: Journal Article
Abstract: Optical clearing methods can facilitate deep optical imaging in biological tissue by reducing light scattering and this has enabled accurate three-dimensional signal visualization and quantification of complex biological structures. Unfortunately, existing optical clearing approaches present a compromise between maximizing clearing capability, the preservation of fluorescent protein emission and membrane integrity and the speed of sample processing – with the latter typically requiring weeks for cm scale tissue samples. To address this challenge, we present a new, convenient, aqueous optical clearing agent, termed UbasM: Urea-Based Amino-Sugar Mixture, that rapidly renders fixed tissue samples highly transparent and reliably preserves emission from fluorescent proteins and lipophilic dyes in membrane integrity preserved tissues. UbasM is simple, inexpensive, reproducible and compatible with all labeling methods that we have encountered. It can enable convenient, volumetric imaging of tissue up to the scale of whole adult mouse organs and should be useful for a wide range of light microscopy and tomography techniques applied to biomedical research, especially the study on organism-level systems biology at multiple levels.
Issue Date: 22-Sep-2017
Date of Acceptance: 8-Sep-2017
URI: http://hdl.handle.net/10044/1/69705
DOI: https://dx.doi.org/10.1038/s41598-017-12484-3
ISSN: 2045-2322
Publisher: Nature Publishing Group
Journal / Book Title: Scientific Reports
Volume: 7
Issue: 1
Copyright Statement: © 2017 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
LIGHT-SHEET MICROSCOPY
MOUSE-BRAIN
ILLUMINATION MICROSCOPY
FLUORESCENCE MICROSCOPY
EMBRYONIC-DEVELOPMENT
PLANE ILLUMINATION
NERVOUS-SYSTEM
EXPRESSION
RECONSTRUCTION
TOMOGRAPHY
Publication Status: Published
Article Number: 12218
Online Publication Date: 2017-09-22
Appears in Collections:Physics
Photonics
Faculty of Natural Sciences



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