Altmetric

Bat and pig IFN-induced transmembrane protein 3 restrict cell entry by influenza virus and lyssaviruses

Title: Bat and pig IFN-induced transmembrane protein 3 restrict cell entry by influenza virus and lyssaviruses
Authors: Benfield, CTO
Smith, SE
Wright, E
Wash, RS
Ferrara, F
Temperton, NJ
Kellam, P
Item Type: Journal Article
Abstract: IFN-induced transmembrane protein 3 (IFITM3) is a restriction factor that blocks cytosolic entry of numerous viruses that utilize acidic endosomal entry pathways. In humans and mice, IFITM3 limits influenza-induced morbidity and mortality. Although many IFITM3-sensitive viruses are zoonotic, whether IFITMs function as antiviral restriction factors in mammalian species other than humans and mice is unknown. Here, IFITM3 orthologues in the microbat (Myotis myotis) and pig (Sus scrofa domesticus) were identified using rapid amplification of cDNA ends. Amino acid residues known to be important for IFITM3 function were conserved in the pig and microbat orthologues. Ectopically expressed pig and microbat IFITM3 co-localized with transferrin (early endosomes) and CD63 (late endosomes/multivesicular bodies). Pig and microbat IFITM3 restricted cell entry mediated by multiple influenza haemagglutinin subtypes and lyssavirus glycoproteins. Expression of pig or microbat IFITM3 in A549 cells reduced influenza virus yields and nucleoprotein expression. Conversely, small interfering RNA knockdown of IFITM3 in pig NPTr cells and primary microbat cells enhanced virus replication, demonstrating that these genes are functional in their species of origin at endogenous levels. In summary, we showed that IFITMs function as potent broad-spectrum antiviral effectors in two mammals – pigs and bats – identified as major reservoirs for emerging viruses.
Issue Date: 1-May-2015
Date of Acceptance: 14-Jan-2015
URI: http://hdl.handle.net/10044/1/69570
DOI: https://dx.doi.org/10.1099/vir.0.000058
ISSN: 1465-2099
Publisher: Microbiology Society
Start Page: 991
End Page: 1005
Journal / Book Title: Journal of General Virology
Volume: 96
Issue: 5
Copyright Statement: © 2015 The Authors. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/).
Keywords: Science & Technology
Life Sciences & Biomedicine
Biotechnology & Applied Microbiology
Virology
GENE-EXPRESSION
IFITM PROTEINS
ANTIVIRAL ACTIVITY
LENTIVIRAL VECTOR
EMERGING VIRUSES
S-PALMITOYLATION
DENGUE VIRUS
VIRAL ENTRY
INTERFERON
INFECTION
Animals
Chiroptera
Conserved Sequence
Interferons
Lyssavirus
Membrane Proteins
Orthomyxoviridae
RNA-Binding Proteins
Sequence Homology, Amino Acid
Swine
Virus Internalization
06 Biological Sciences
07 Agricultural and Veterinary Sciences
11 Medical and Health Sciences
Publication Status: Published
Online Publication Date: 2015-05-01
Appears in Collections:Department of Medicine
Faculty of Medicine



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Creative Commons