A membrane topology model for human interferon inducible transmembrane protein 1

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Title: A membrane topology model for human interferon inducible transmembrane protein 1
Authors: Weston, S
Czieso, S
White, IJ
Smith, SE
Kellam, P
Marsh, M
Item Type: Journal Article
Abstract: InterFeron Inducible TransMembrane proteins 1–3 (IFITM1, IFITM2 and IFITM3) are a family of proteins capable of inhibiting the cellular entry of numerous human and animal viruses. IFITM1-3 are unique amongst the currently described viral restriction factors in their apparent ability to block viral entry. This restrictive property is dependant on the localisation of the proteins to plasma and endosomal membranes, which constitute the main portals of viral entry into cells. The topology of the IFITM proteins within cell membranes is an unresolved aspect of their biology. Here we present data from immunofluorescence microscopy, protease cleavage, biotin-labelling and immuno-electron microscopy assays, showing that human IFITM1 has a membrane topology in which the N-terminal domain resides in the cytoplasm, and the C-terminal domain is extracellular. Furthermore, we provide evidence that this topology is conserved for all of the human interferon-induced IFITM proteins. This model is consistent with that recently proposed for murine IFITM3, but differs from that proposed for murine IFITM1.
Issue Date: 8-Aug-2014
Date of Acceptance: 13-Jul-2014
ISSN: 1932-6203
Publisher: Public Library of Science (PLoS)
Journal / Book Title: PLoS ONE
Volume: 9
Issue: 8
Copyright Statement: © 2014 Weston et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
Antigens, Differentiation
Blotting, Western
Cell Line, Tumor
DNA Primers
Flow Cytometry
Fluorescent Antibody Technique
HEK293 Cells
Image Processing, Computer-Assisted
Microscopy, Electron, Transmission
Models, Molecular
Protein Conformation
Real-Time Polymerase Chain Reaction
MD Multidisciplinary
General Science & Technology
Publication Status: Published
Article Number: e104341
Online Publication Date: 2014-08-08
Appears in Collections:Department of Medicine

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