Hyperspectral scanning laser optical tomography

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Title: Hyperspectral scanning laser optical tomography
Authors: Chen, L
Li, G
Tang, L
Zhang, M
Liu, L
Liu, A
McGinty, J
Ruan, S
Item Type: Journal Article
Abstract: In order to study physical relationships within tissue volumes or even organism-level systems, the spatial distribution of multiple fluorescent markers needs to be resolved efficiently in three dimensions. Here, rather than acquiring discrete spectral images sequentially using multiple emission filters, a hyperspectral scanning laser optical tomography system is developed to obtain hyperspectral volumetric data sets with 2-nm spectral resolution of optically transparent mesoscopic (millimeter-centimeter) specimens. This is achieved by acquiring a series of point-scanning hyperspectral extended depth of field images at different angles and subsequently tomographically reconstructing the 3D intensity distribution for each wavelength. This technique is demonstrated to provide robust measurements via the comparison of spectral and intensity profiles of fluorescent bead phantoms. Due to its enhanced spectral resolving ability, this technique is also demonstrated to resolve largely overlapping fluorophores, as demonstrated by the 3D fluorescence hyperspectral reconstruction of a dual-labeled mouse thymus gland sample and the ability to distinguish tumorous and normal tissues of an unlabeled mouse intestine sample.
Issue Date: 1-Apr-2019
Date of Acceptance: 4-Sep-2018
URI: http://hdl.handle.net/10044/1/68453
DOI: https://dx.doi.org/10.1002/jbio.201800221
ISSN: 1864-063X
Publisher: Wiley-VCH Verlag
Journal / Book Title: Journal of Biophotonics
Volume: 12
Issue: 4
Copyright Statement: © 2018 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is the pre-peer reviewed version of the following article, which has been published in final form at https://onlinelibrary.wiley.com/doi/full/10.1002/jbio.201800221
Keywords: fluorescence
hyperspectral imaging
mesoscopic sample
three-dimensional imaging
Optoelectronics & Photonics
Publication Status: Published
Conference Place: Germany
Article Number: e201800221
Online Publication Date: 2018-09-05
Appears in Collections:Physics

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