Buoyancy-driven gradients for biomaterial fabrication and tissue engineering

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Title: Buoyancy-driven gradients for biomaterial fabrication and tissue engineering
Authors: Li, C
Ouyang, L
Pence, I
Moore, A
Lin, Y
Winter, C
Armstrong, J
Stevens, M
Item Type: Journal Article
Abstract: The controlled fabrication of gradient materials is becoming increasingly important as the next generation of tissue engineering seeks to produce inhomogeneous constructs with physiological complexity. Current strategies for fabricating gradient materials can require highly specialized materials or equipment and cannot be generally applied to the wide range of systems used for tissue engineering. Here, the fundamental physical principle of buoyancy is exploited as a generalized approach for generating materials bearing well‐defined compositional, mechanical, or biochemical gradients. Gradient formation is demonstrated across a range of different materials (e.g., polymers and hydrogels) and cargos (e.g., liposomes, nanoparticles, extracellular vesicles, macromolecules, and small molecules). As well as providing versatility, this buoyancy‐driven gradient approach also offers speed (<1 min) and simplicity (a single injection) using standard laboratory apparatus. Moreover, this technique is readily applied to a major target in complex tissue engineering: the osteochondral interface. A bone morphogenetic protein 2 gradient, presented across a gelatin methacryloyl hydrogel laden with human mesenchymal stem cells, is used to locally stimulate osteogenesis and mineralization in order to produce integrated osteochondral tissue constructs. The versatility and accessibility of this fabrication platform should ensure widespread applicability and provide opportunities to generate other gradient materials or interfacial tissues.
Issue Date: 25-Apr-2019
Date of Acceptance: 19-Feb-2019
URI: http://hdl.handle.net/10044/1/67996
DOI: https://doi.org/10.1002/adma.201900291
ISSN: 0935-9648
Publisher: Wiley
Journal / Book Title: Advanced Materials
Volume: 31
Issue: 17
Copyright Statement: © 2019 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Sponsor/Funder: Engineering & Physical Science Research Council (E
Commission of the European Communities
Medical Research Council (MRC)
Wellcome Trust
Arthritis Research UK
Medical Research Council (MRC)
Biotechnology and Biological Sciences Research Council
Medical Research Council
Arthritis Research UK
Funder's Grant Number: 20069192
ERC-2013-CoG-616417
MR/R015651/1
098411/Z/12/Z
21138
MR/S00551X/1
BB/N503952/1
MR/S00551X/1
21138
Keywords: Science & Technology
Physical Sciences
Technology
Chemistry, Multidisciplinary
Chemistry, Physical
Nanoscience & Nanotechnology
Materials Science, Multidisciplinary
Physics, Applied
Physics, Condensed Matter
Chemistry
Science & Technology - Other Topics
Materials Science
Physics
biomaterials
buoyancy
gradients
osteochondral
tissue engineering
ARTICULAR-CARTILAGE
NANOPARTICLES
NANOFIBERS
SCAFFOLDS
PROTEIN
biomaterials
buoyancy
gradients
osteochondral
tissue engineering
Nanoscience & Nanotechnology
02 Physical Sciences
03 Chemical Sciences
09 Engineering
Publication Status: Published
Article Number: 1900291
Online Publication Date: 2019-03-07
Appears in Collections:Faculty of Engineering
Materials
Faculty of Natural Sciences



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