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DamID as a versatile tool for understanding gene regulation

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Title: DamID as a versatile tool for understanding gene regulation
Authors: Southall, T
Aughey, G
Cheetham, S
Item Type: Journal Article
Abstract: The interaction of proteins and RNA with chromatin underlies the regulation of gene expression. The ability to profile easily these interactions is fundamental for understanding chromatin biology in vivo. DNA adenine methyltransferase identification (DamID) profiles genome-wide protein-DNA interactions without antibodies, fixation or protein pull-downs. Recently, DamID has been adapted for applications beyond simple assaying of protein-DNA interactions, such as for studying RNA-chromatin interactions, chromatin accessibility and long-range chromosome interactions. Here, we provide an overview of DamID and introduce improvements to the technology, discuss their applications and compare alternative methodologies.
Issue Date: 15-Mar-2019
Date of Acceptance: 18-Feb-2019
URI: http://hdl.handle.net/10044/1/67976
DOI: https://dx.doi.org/10.1242/dev.173666
ISSN: 0950-1991
Publisher: Company of Biologists Ltd
Journal / Book Title: Development
Volume: 146
Issue: 6
Copyright Statement: © 2019. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/4.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
Sponsor/Funder: Wellcome Trust
Biotechnology and Biological Sciences Research Council (BBSRC)
Funder's Grant Number: 104567/Z/14/Z
BB/P017924/1
Keywords: Chromatin
DamID
Gene regulation
Genomics
06 Biological Sciences
11 Medical And Health Sciences
Publication Status: Published
Article Number: dev173666
Appears in Collections:Faculty of Natural Sciences



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