Measuring intracellular viscosity in conditions of hypergravity

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Title: Measuring intracellular viscosity in conditions of hypergravity
Authors: Woodcock, EM
Girvan, P
Eckert, J
Lopez Duarte, I
Kubankova, M
Van Loon, J
Brooks, N
Kuimova, M
Item Type: Journal Article
Abstract: Gravity-sensitive cellular responses are regularly observed in both specialised and non-specialised cells. One potential mechanism for this sensitivity is a changing viscosity of the intracellular organelles. Here, we report a novel viscosity-sensitive molecular rotor based on meso-substituted boron-dipyrrin (BODIPY) used to investigate the response of viscosity of cellular membranes to hypergravity conditions created at the Large Diameter Centrifuge at the European Space Agency Technology Centre ESA-ESTEC. Mouse osteoblastic (MC3T3-E1) and endothelial (HUVEC) cell lines were tested and an increase in viscosity was found with increasing hypergravity loading. This response is thought to be primarily biologically driven, with the potential for a small, instantaneous physical mechanism also contributing to the observed effect. This work provides the first quantitative data for cellular viscosity changes under hypergravity, up to 15 g.
Issue Date: 8-Apr-2019
Date of Acceptance: 19-Mar-2019
URI: http://hdl.handle.net/10044/1/67828
ISSN: 0006-3495
Publisher: Biophysical Society
Journal / Book Title: Biophysical Journal
Copyright Statement: This paper is embargoed until 12 months after publication.
Sponsor/Funder: Engineering & Physical Science Research Council (EPSRC)
Funder's Grant Number: EP/J017566/1
Keywords: 02 Physical Sciences
03 Chemical Sciences
06 Biological Sciences
Biophysics
Publication Status: Accepted
Embargo Date: 2020-04-08
Appears in Collections:Chemistry
Biological and Biophysical Chemistry



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