Altmetric

High-throughput sequencing in respiratory, critical care, and sleep medicine research. An Official American Thoracic Society Workshop Report

File Description SizeFormat 
Workshop report - version.docxAccepted version786.41 kBMicrosoft WordView/Open
Title: High-throughput sequencing in respiratory, critical care, and sleep medicine research. An Official American Thoracic Society Workshop Report
Authors: Hersh, CP
Adcock, IM
Celedon, JC
Cho, MH
Christiani, DC
Himes, BE
Kaminski, N
Mathias, RA
Meyers, DA
Quackenbush, J
Redline, S
Steiling, KA
Tabor, HK
Tobin, MD
Wurfel, MM
Yang, IV
Koppelman, GH
Item Type: Journal Article
Abstract: High-throughput, “next-generation” sequencing methods are now being broadly applied across all fields of biomedical research, including respiratory disease, critical care, and sleep medicine. Although there are numerous review articles and best practice guidelines related to sequencing methods and data analysis, there are fewer resources summarizing issues related to study design and interpretation, especially as applied to common, complex, nonmalignant diseases. To address these gaps, a single-day workshop was held at the American Thoracic Society meeting in May 2017, led by the American Thoracic Society Section on Genetics and Genomics. The aim of this workshop was to review the design, analysis, interpretation, and functional follow-up of high-throughput sequencing studies in respiratory, critical care, and sleep medicine research. This workshop brought together experts in multiple fields, including genetic epidemiology, biobanking, bioinformatics, and research ethics, along with physician-scientists with expertise in a range of relevant diseases. The workshop focused on application of DNA and RNA sequencing research in common chronic diseases and did not cover sequencing studies in lung cancer, monogenic diseases (e.g., cystic fibrosis), or microbiome sequencing. Participants reviewed and discussed study design, data analysis and presentation, interpretation, functional follow-up, and reporting of results. This report summarizes the main conclusions of the workshop, specifically addressing the application of these methods in respiratory, critical care, and sleep medicine research. This workshop report may serve as a resource for our research community as well as for journal editors and reviewers of sequencing-based manuscript submissions in our research field.
Issue Date: 1-Jan-2019
Date of Acceptance: 19-Dec-2018
URI: http://hdl.handle.net/10044/1/67691
DOI: https://dx.doi.org/10.1513/AnnalsATS.201810-716WS
ISSN: 2329-6933
Publisher: American Thoracic Society
Start Page: 1
End Page: 16
Journal / Book Title: Annals of the American Thoracic Society
Volume: 16
Issue: 1
Copyright Statement: ©2019 by the American Thoracic Society
Keywords: Science & Technology
Life Sciences & Biomedicine
Respiratory System
bioinformatics
functional genomics
genetic epidemiology
RNA sequencing
whole-genome sequencing
GENOME-WIDE ASSOCIATION
OBSTRUCTIVE PULMONARY-DISEASE
DIFFERENTIALLY EXPRESSED GENES
RNA-SEQ DATA
LUNG-FUNCTION
DISTRESS-SYNDROME
QUALITY-CONTROL
READ ALIGNMENT
EXOME
GENERATION
Publication Status: Published
Online Publication Date: 2019-01-01
Appears in Collections:National Heart and Lung Institute
Airway Disease



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Creative Commons