Bio-inspired fabrication of DNA-inorganic hybrid composites using synthetic DNA

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Title: Bio-inspired fabrication of DNA-inorganic hybrid composites using synthetic DNA
Authors: Kim, E
Agarwal, S
Kim, N
Hage, FS
Gelmi, A
Stevens, M
Item Type: Journal Article
Abstract: Nucleic acid nanostructures have attracted significant interest as potential therapeutic and diagnostic platforms due to their intrinsic biocompatibility and biodegradability, structural and functional diversity, and compatibility with various chemistries for modification and stabilization. Among the fabrication approaches for such structures, the rolling circle techniques have emerged as particularly promising, producing morphologically round, flower-shaped nucleic acid particles: typically hybrid composites of long nucleic acid strands and inorganic magnesium pyrophosphate (Mg2PPi). These constructs are known to form via anisotropic nucleic acid-driven crystallization in a sequence-independent manner, rendering monodisperse and densely packed RNA or DNA–inorganic composites. However, it still remains to fully explore how flexible polymer-like RNA or DNA strands (acting as biomolecular additives) mediate the crystallization process of Mg2PPi and affect the structure and properties of the product crystals. To address this, we closely examined nanoscale details to mesoscopic features of Mg2PPi/DNA hybrid composites fabricated by two approaches, namely rolling circle amplification (RCA)-based in situ synthesis and long synthetic DNA-mediated crystallization. Similar to the DNA constructs fabricated by RCA, the rapid crystallization of Mg2PPi was retarded on a short-range order when we precipitated the crystals in the presence of presynthesized long DNA, which resulted in effective incorporation of biomolecular additives such as DNA and enzymes. These findings further provide a more feasible way to encapsulate bioactive enzymes within DNA constructs compared to in situ RCA-mediated synthesis, i.e., by not only protecting them from possible denaturation under the reaction conditions but also preventing nonselective association of proteins arising from the RCA reaction mixtures.
Issue Date: 26-Mar-2019
Date of Acceptance: 6-Feb-2019
URI: http://hdl.handle.net/10044/1/67407
DOI: https://doi.org/10.1021/acsnano.8b06492
ISSN: 1936-0851
Publisher: American Chemical Society
Start Page: 2888
End Page: 2900
Journal / Book Title: ACS Nano
Volume: 13
Issue: 3
Copyright Statement: © 2019 American Chemical Society. This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS NANO, after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acsnano.8b06492
Sponsor/Funder: Commission of the European Communities
Commission of the European Communities
Wellcome Trust
Engineering & Physical Science Research Council (EPSRC)
Engineering & Physical Science Research Council (E
Funder's Grant Number: 660757
ERC-2013-CoG-616417
098411/Z/12/Z
EP/K020641/1
EP/K031953/1
Keywords: Science & Technology
Physical Sciences
Technology
Chemistry, Multidisciplinary
Chemistry, Physical
Nanoscience & Nanotechnology
Materials Science, Multidisciplinary
Chemistry
Science & Technology - Other Topics
Materials Science
DNA-inorganic hybrid composites
rolling circle techniques
crystallization
coprecipitation
DNA inclusion
ELECTRON-MICROSCOPY REVEALS
SINGLE-STRANDED-DNA
RAMAN-SPECTROSCOPY
CRYSTAL-STRUCTURE
METAL COMPLEXES
AMPLIFICATION
XANES
MICROSTRUCTURE
MICROSPONGES
NANOFLOWERS
DNA inclusion
DNA-inorganic hybrid composites
coprecipitation
crystallization
rolling circle techniques
Nanoscience & Nanotechnology
Publication Status: Published
Embargo Date: 2020-02-11
Online Publication Date: 2019-02-11
Appears in Collections:Faculty of Engineering
Materials
Faculty of Natural Sciences



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