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Chromosome translocation inflates bacillus forespores and impacts cellular morphology

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Title: Chromosome translocation inflates bacillus forespores and impacts cellular morphology
Authors: Lopez-Garrido, J
Ojkic, N
Khanna, K
Wagner, FR
Villa, E
Endres, RG
Pogliano, K
Item Type: Journal Article
Abstract: The means by which the physicochemical properties of different cellular components together determine bacterial cell shape remain poorly understood. Here, we investigate a programmed cell-shape change during Bacillus subtilis sporulation, when a rod-shaped vegetative cell is transformed to an ovoid spore. Asymmetric cell division generates a bigger mother cell and a smaller, hemispherical forespore. The septum traps the forespore chromosome, which is translocated to the forespore by SpoIIIE. Simultaneously, forespore size increases as it is reshaped into an ovoid. Using genetics, timelapse microscopy, cryo-electron tomography, and mathematical modeling, we demonstrate that forespore growth relies on membrane synthesis and SpoIIIE-mediated chromosome translocation, but not on peptidoglycan or protein synthesis. Our data suggest that the hydrated nucleoid swells and inflates the forespore, displacing ribosomes to the cell periphery, stretching septal peptidoglycan, and reshaping the forespore. Our results illustrate how simple biophysical interactions between core cellular components contribute to cellular morphology.
Issue Date: 8-Feb-2018
Date of Acceptance: 18-Jan-2018
URI: http://hdl.handle.net/10044/1/67348
DOI: https://dx.doi.org/10.1016/j.cell.2018.01.027
ISSN: 0092-8674
Publisher: Elsevier (Cell Press)
Start Page: 758
End Page: 770.e14
Journal / Book Title: Cell
Volume: 172
Issue: 4
Copyright Statement: © 2018 Elsevier Ltd. All rights reserved. This manuscript is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence http://creativecommons.org/licenses/by-nc-nd/4.0/
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Cell Biology
SUBTILIS SPORULATION
ESCHERICHIA-COLI
CRYOELECTRON TOMOGRAPHY
MEMBRANE DYNAMICS
DNA TRANSLOCATION
OSMOTIC-PRESSURE
BACTERIAL SHAPE
SPOIIIE
GROWTH
DIVISION
Bacillus subtilis
Gibbs/Donnan effect
Langevin simulations
SpoIIIE
cell shape
cell size
chromosome translocation
cryo-electron tomography
sporulation
turgor pressure
Asymmetric Cell Division
Bacterial Proteins
Chromosomes, Bacterial
Peptidoglycan
Protein Biosynthesis
Spores, Bacterial
Translocation, Genetic
06 Biological Sciences
11 Medical And Health Sciences
Developmental Biology
Publication Status: Published
Online Publication Date: 2018-02-08
Appears in Collections:Faculty of Natural Sciences



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