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Single-neuron level one-photon voltage imaging with sparsely targeted genetically encoded voltage indicators

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Title: Single-neuron level one-photon voltage imaging with sparsely targeted genetically encoded voltage indicators
Authors: Quicke, P
Song, C
McKimm, EJ
Milosevic, MM
Howe, CL
Neil, M
Schultz, SR
Antic, SD
Foust, AJ
Knopfel, T
Item Type: Journal Article
Abstract: Voltage imaging of many neurons simultaneously at single-cell resolution is hampered by the difficulty of detecting small voltage signals from overlapping neuronal processes in neural tissue. Recent advances in genetically encoded voltage indicator (GEVI) imaging have shown single-cell resolution optical voltage recordings in intact tissue through imaging naturally sparse cell classes, sparse viral expression, soma restricted expression, advanced optical systems, or a combination of these. Widespread sparse and strong transgenic GEVI expression would enable straightforward optical access to a densely occurring cell type, such as cortical pyramidal cells. Here we demonstrate that a recently described sparse transgenic expression strategy can enable single-cell resolution voltage imaging of cortical pyramidal cells in intact brain tissue without restricting expression to the soma. We also quantify the functional crosstalk in brain tissue and discuss optimal imaging rates to inform future GEVI experimental design.
Issue Date: 14-Feb-2019
Date of Acceptance: 24-Jan-2019
URI: http://hdl.handle.net/10044/1/67259
DOI: https://dx.doi.org/10.3389/fncel.2019.00039
ISSN: 1662-5102
Publisher: Frontiers Media
Journal / Book Title: Frontiers in Cellular Neuroscience
Volume: 13
Copyright Statement: © 2019 Quicke, Song, McKimm, Milosevic, Howe, Neil, Schultz, Antic, Foust and Knöpfel. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY, https://creativecommons.org/licenses/by/4.0/). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Sponsor/Funder: Biotechnology and Biological Sciences Research Council (BBSRC)
Engineering & Physical Science Research Council (E
Royal Academy Of Engineering
National Institutes of Health
National Institutes of Health
Wellcome Trust
Biotechnology and Biological Sciences Research Council (BBSRC)
The Royal Society
Funder's Grant Number: BB/K001817/1
EP/K503733/1
RF1415\14\26
UPMC: C15/0244
UCHC7-91739613-A2
201964/Z/16/Z
BB/R009007/1
TA/R1/170047
Keywords: Science & Technology
Life Sciences & Biomedicine
Neurosciences
Neurosciences & Neurology
voltage imaging
cerebral cortex
sparse expression
optogenetics
transgenic
CIRCUIT DYNAMICS
MAMMALIAN-CELLS
GENE-EXPRESSION
FLUORESCENT
EXCITATION
MICE
Publication Status: Published
Article Number: 39
Appears in Collections:Physics
Bioengineering
Photonics
Department of Medicine
Faculty of Medicine
Faculty of Natural Sciences



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