Towards multiplexed near-infrared cellular imaging using gold nanostar arrays with tunable fluorescence enhancement

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Title: Towards multiplexed near-infrared cellular imaging using gold nanostar arrays with tunable fluorescence enhancement
Authors: Theodorou, I
Ruenraroengsak, P
Carter, D
Jiang, Q
Yague, E
Aboagye, E
Coombes, RC
Porter, AE
Ryan, M
Xie, F
Item Type: Journal Article
Abstract: Sensitive detection of disease biomarkers expressed by human cells is critical to the development of novel diagnostic and therapeutic methods. Here we report that plasmonic arrays based on gold nanostar (AuNS) monolayers enable up to 19-fold fluorescence enhancement for cellular imaging in the near-infrared (NIR) biological window, allowing the application of low quantum yield fluorophores for sensitive cellular imaging. The high fluorescence enhancement together with low autofluorescence interference in this wavelength range enable higher signal-to-noise ratio compared to other diagnostic modalities. Using AuNSs of different geometries and therefore controllable electric field enhancement, cellular imaging with tunable enhancement factors is achieved, which may be useful for the development of multicolour and multiplexed platforms for a panel of biomarkers, allowing to distinguish different subcell populations at the single cell level. Finally, the uptake of AuNSs within HeLa cells and their high biocompatibility, pave the way for novel high-performance in vitro and in vivo diagnostic platforms.
Issue Date: 28-Jan-2019
Date of Acceptance: 5-Jan-2019
URI: http://hdl.handle.net/10044/1/66967
DOI: https://dx.doi.org/10.1039/C8NR09409H
ISSN: 2040-3364
Publisher: Royal Society of Chemistry
Start Page: 2079
End Page: 2088
Journal / Book Title: Nanoscale
Volume: 11
Copyright Statement: © The Royal Society of Chemistry 2019
Sponsor/Funder: Cancer Research UK
Royal Academy Of Engineering
British Council
Funder's Grant Number: C2536/A16584
MMRE_P56611
216239013
Keywords: 10 Technology
02 Physical Sciences
03 Chemical Sciences
Nanoscience & Nanotechnology
Publication Status: Published
Embargo Date: 2020-01-07
Online Publication Date: 2019-01-07
Appears in Collections:Faculty of Engineering
Materials
Division of Surgery
Division of Cancer
Faculty of Medicine
Faculty of Natural Sciences



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