Application of novel solid phase extraction-NMR protocols for metabolic profiling of human urine

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Title: Application of novel solid phase extraction-NMR protocols for metabolic profiling of human urine
Authors: McGill, D
Chekmeneva, E
Lindon, J
Takats, Z
Nicholson, J
Item Type: Journal Article
Abstract: Metabolite identification and annotation procedures are necessary for the discovery of biomarkers indicative of phenotypes or disease states, but these processes can be bottlenecked by the sheer complexity of biofluids containing thousands of different compounds. Here we describe low-cost novel SPE-NMR protocols utilising different cartridges and conditions, on both natural and artifical urine mixtures, which produce unique retention profiles useful to metabolic profiling. We find that different SPE methods applied to biofluids such as urine can be used to selectively retain metabolites based on compound taxonomy or other key functional groups, reducing peak overlap through concentration and fractionation of unknowns and hence promising greater control over the metabolite annotation/identification process.
Issue Date: 1-Aug-2019
Date of Acceptance: 6-Dec-2018
URI: http://hdl.handle.net/10044/1/66679
DOI: https://dxdoi.org/10.1039/C8FD00220G
ISSN: 1359-6640
Publisher: Royal Society of Chemistry
Start Page: 395
End Page: 416
Journal / Book Title: Faraday Discussions
Volume: 218
Copyright Statement: This journal is © The Royal Society of Chemistry 2018. This open access article is licensed under a Creative Commons Attribution 3.0 Unported Licence (https://creativecommons.org/licenses/by/3.0/).
Sponsor/Funder: Imperial College Healthcare NHS Trust- BRC Funding
Funder's Grant Number: RDB04
Keywords: Science & Technology
Physical Sciences
Chemistry, Physical
Chemistry
SPECTROSCOPY
IDENTIFICATION
SPE
Clinical Medicine
Analytical Chemistry
0306 Physical Chemistry (incl. Structural)
0904 Chemical Engineering
0303 Macromolecular and Materials Chemistry
Chemical Physics
Publication Status: Published
Conference Place: United Kingdom
Online Publication Date: 2019-01-21
Appears in Collections:Division of Surgery
Faculty of Medicine



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