Bacterial load and defective monocyte-derived macrophage bacterial phagocytosis in biomass-smoke COPD

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Title: Bacterial load and defective monocyte-derived macrophage bacterial phagocytosis in biomass-smoke COPD
Authors: Ghosh, B
Gaike, AH
Pyasi, K
Brashier, B
Das, VV
Londhe, JD
Juvekar, S
Shouche, YS
Donnelly, LE
Salvi, SS
Barnes, PJ
Item Type: Journal Article
Abstract: Lower airway colonisation with potentially pathogenic bacterial species (PPBs) is associated with defective bacterial phagocytosis, in monocyte-derived macrophages (MDMs) and alveolar macrophages, from tobacco-smoke associated COPD (S-COPD) subjects. In developing world, COPD among non-smokers is largely due to biomass-smoke (BMS) exposure. Yet, little is known about PPBs colonisation and its association with impaired innate immunity in these subjects.We investigated the PPBs load (Streptococcus pneumoniae, SP; Haemophilus influenzae, HI; Moraxella catarrhalis, MC; and Pseudomonas aeruginosa, PA) in BMS-exposed COPD (BMS-COPD) compared with S-COPD and spirometrically normal subjects. We also examined the association between load of PPBs with phagocytic activity of MDMs and lung function.Induced sputum and peripheral venous blood samples were collected from 18 healthy non-smokers, 15 smokers without COPD, 16 BMS-exposed healthy, 19 S-COPD and 23 BMS-COPD subjects. PPBs load in induced sputum and MDMs phagocytic activity were determined using qPCR and fluorimetry respectively.Higher bacterial load of SP, HI, and PA were observed in BMS-COPD. Increased PPBs load in BMS-exposed subjects was significantly negatively associated with defective phagocytosis in MDMs, and spirometric lung function indices (p<0.05).Increased load of PPBs in airways of BMS-COPD subjects is inversely associated with defective bacterial phagocytosis and lung function.
Issue Date: 21-Dec-2018
Date of Acceptance: 21-Nov-2018
ISSN: 0903-1936
Publisher: European Respiratory Society
Journal / Book Title: European Respiratory Journal
Copyright Statement: © 2018 ERS. This is an author-submitted, peer-reviewed version of a manuscript that has been accepted for publication in the European Respiratory Journal, prior to copy-editing, formatting and typesetting. This version of the manuscript may not be duplicated or reproduced without prior permission from the copyright owner, the European Respiratory Society. The publisher is not responsible or liable for any errors or omissions in this version of the manuscript or in any version derived from it by any other parties. The final, copy-edited, published article, which is the version of record, is available without a subscription 18 months after the date of issue publication.
Sponsor/Funder: Medical Research Council (MRC)
Funder's Grant Number: MR/J000108/1
Keywords: 11 Medical And Health Sciences
Respiratory System
Publication Status: Published online
Conference Place: England
Embargo Date: 2021-06-21
Online Publication Date: 2018-12-21
Appears in Collections:National Heart and Lung Institute
Airway Disease
Faculty of Medicine

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