Spatiotemporal quantification of acoustic cell patterning using Voronoi Tessellation

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Title: Spatiotemporal quantification of acoustic cell patterning using Voronoi Tessellation
Authors: Armstrong, J
Maynard, S
Pence, I
Franklin, AC
Drinkwater, BW
Stevens, M
Item Type: Journal Article
Abstract: Acoustic patterning using ultrasound standing waves has recently emerged as a potent biotechnology enabling the remote generation of ordered cell systems. This capability has opened up exciting opportunities, for example, in guiding the development of organoid cultures or the organization of complex tissues. The success of these studies is often contingent on the formation of tightly-packed and uniform cell arrays; however, a number of factors can act to disrupt or prevent acoustic patterning. Yet, to the best of our knowledge, there has been no comprehensive assessment of the quality of acoustically-patterned cell populations. In this report we use a mathematical approach, known as Voronoï tessellation, to generate a series of metrics that can be used to measure the effect of cell concentration, pressure amplitude, ultrasound frequency and biomaterial viscosity upon the quality of acoustically-patterned cell systems. Moreover, we extend this approach towards the characterization of spatiotemporal processes, namely, the acoustic patterning of cell suspensions and the migration of patterned, adherent cell clusters. This strategy is simple, unbiased and highly informative, and we anticipate that the methods described here will provide a systematic framework for all stages of acoustic patterning, including the robust quality control of devices, statistical comparison of patterning conditions, the quantitative exploration of parameter limits and the ability to track patterned tissue formation over time.
Issue Date: 21-Feb-2019
Date of Acceptance: 30-Nov-2018
URI: http://hdl.handle.net/10044/1/66598
DOI: https://dx.doi.org/10.1039/C8LC01108G
ISSN: 1473-0189
Publisher: Royal Society of Chemistry
Start Page: 562
End Page: 573
Journal / Book Title: Lab on a Chip
Volume: 19
Issue: 4
Copyright Statement: This journal is © The Royal Society of Chemistry 2019. This open access article is licensed under a Creative Commons Attribution 3.0 Unported Licence (https://creativecommons.org/licenses/by/3.0/).
Sponsor/Funder: Medical Research Council (MRC)
Wellcome Trust
Commission of the European Communities
Medical Research Council (MRC)
Medical Research Council (MRC)
Arthritis Research UK
Funder's Grant Number: MR/K026682/1
098411/Z/12/Z
ERC-2013-CoG-616417
MR/R015651/1
MR/S00551X/1
21138
Keywords: 03 Chemical Sciences
09 Engineering
Analytical Chemistry
Publication Status: Published
Online Publication Date: 2019-01-22
Appears in Collections:Faculty of Engineering
Materials
Faculty of Natural Sciences



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