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Inhaled Nanoformulated mRNA Polyplexes for Protein Production in Lung Epithelium

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Title: Inhaled Nanoformulated mRNA Polyplexes for Protein Production in Lung Epithelium
Authors: Patel, AK
Kaczmarek, JC
Bose, S
Kauffman, KJ
Mir, F
Heartlein, MW
DeRosa, F
Langer, R
Anderson, DG
Item Type: Journal Article
Abstract: Noninvasive aerosol inhalation is an established method of drug delivery to the lung, and remains a desirable route for nucleic‐acid‐based therapeutics. In vitro transcribed (IVT) mRNA has broad therapeutic applicability as it permits temporal and dose‐dependent control of encoded protein expression. Inhaled delivery of IVT‐mRNA has not yet been demonstrated and requires development of safe and effective materials. To meet this need, hyperbranched poly(beta amino esters) (hPBAEs) are synthesized to enable nanoformulation of stable and concentrated polyplexes suitable for inhalation. This strategy achieves uniform distribution of luciferase mRNA throughout all five lobes of the lung and produces 101.2 ng g−1 of luciferase protein 24 h after inhalation of hPBAE polyplexes. Importantly, delivery is localized to the lung, and no luminescence is observed in other tissues. Furthermore, using an Ai14 reporter mouse model it is identified that 24.6% of the total lung epithelial cell population is transfected after a single dose. Repeat dosing of inhaled hPBAE‐mRNA generates consistent protein production in the lung, without local or systemic toxicity. The results indicate that nebulized delivery of IVT‐mRNA facilitated by hPBAE vectors may provide a clinically relevant delivery system to lung epithelium.
Issue Date: 4-Jan-2019
Date of Acceptance: 29-Nov-2018
URI: http://hdl.handle.net/10044/1/66597
DOI: https://dx.doi.org/10.1002/adma.201805116
ISSN: 0935-9648
Publisher: Wiley
Start Page: 1805116
End Page: 1805116
Journal / Book Title: Advanced Materials
Copyright Statement: © 2019 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is the pre-peer reviewed version of the following article, which has been published in final form at https://onlinelibrary.wiley.com/doi/full/10.1002/adma.201805116
Keywords: biomaterials
gene delivery
inhalation
messenger RNA
topology
02 Physical Sciences
03 Chemical Sciences
09 Engineering
Nanoscience & Nanotechnology
Publication Status: Published online
Embargo Date: 2020-01-04
Online Publication Date: 2019-01-04
Appears in Collections:National Heart and Lung Institute
Faculty of Medicine



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