Activatable cell-biomaterial interfacing with photo-caged peptides

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Title: Activatable cell-biomaterial interfacing with photo-caged peptides
Authors: Lin, Y
Mazo, M
Skaalure, S
Thomas, MR
Schultz, SR
Stevens, M
Item Type: Journal Article
Abstract: Spatio-temporally tailoring cell–material interactions is essential for developing smart delivery systems and intelligent biointerfaces. Here we report new photo-activatable cell–material interfacing systems that trigger cellular uptake of various cargoes and cell adhesion towards surfaces. To achieve this, we designed a novel photo-caged peptide which undergoes a structural transition from an antifouling ligand to a cell-penetrating peptide upon photo-irradiation. When the peptide is conjugated to ligands of interest, we demonstrate the photo-activated cellular uptake of a wide range of cargoes, including small fluorophores, proteins, inorganic (e.g., quantum dots and gold nanostars) and organic nanomaterials (e.g., polymeric particles), and liposomes. Using this system, we can remotely regulate drug administration into cancer cells by functionalizing camptothecin-loaded polymeric nanoparticles with our synthetic peptide ligands. Furthermore, we show light-controlled cell adhesion on a peptide-modified surface and 3D spatiotemporal control over cellular uptake of nanoparticles using two-photon excitation. We anticipate that the innovative approach proposed in this work will help to establish new stimuli-responsive delivery systems and biomaterials.
Issue Date: 28-Jan-2019
Date of Acceptance: 6-Nov-2018
URI: http://hdl.handle.net/10044/1/66279
DOI: https://dx.doi.org/10.1039/C8SC04725A
ISSN: 2041-6520
Publisher: Royal Society of Chemistry
Start Page: 1158
End Page: 1167
Journal / Book Title: Chemical Science
Volume: 10
Copyright Statement: © The Royal Society of Chemistry 2019. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (https://creativecommons.org/licenses/by/3.0/)
Sponsor/Funder: Engineering & Physical Science Research Council (E
Wellcome Trust
Commission of the European Communities
Commission of the European Communities
Commission of the European Communities
Funder's Grant Number: EP/K031953/1
098411/Z/12/Z
ERC-2013-CoG-616417
PIEF-GA-2013-625472
701664
Publication Status: Published
Online Publication Date: 2018-11-16
Appears in Collections:Faculty of Engineering
Materials
Bioengineering
Faculty of Natural Sciences



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