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In vivo multiplex molecular imaging of vascular inflammation using surface-enhanced Raman spectroscopy

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Title: In vivo multiplex molecular imaging of vascular inflammation using surface-enhanced Raman spectroscopy
Authors: Noonan, J
Asiala, SM
Grassia, G
MacRitchie, N
Gracie, K
Carson, J
Moores, M
Girolami, M
Bradshaw, AC
Guzik, TJ
Meehan, GR
Scales, HE
Brewer, JM
McInnes, IB
Sattar, N
Faulds, K
Garside, P
Graham, D
Maffia, P
Item Type: Journal Article
Abstract: Vascular immune-inflammatory responses play a crucial role in the progression and outcome of atherosclerosis. The ability to assess localized inflammation through detection of specific vascular inflammatory biomarkers would significantly improve cardiovascular risk assessment and management; however, no multi-parameter molecular imaging technologies have been established to date. Here, we report the targeted in vivo imaging of multiple vascular biomarkers using antibody-functionalized nanoparticles and surface-enhanced Raman scattering (SERS). Methods: A series of antibody-functionalized gold nanoprobes (BFNP) were designed containing unique Raman signals in order to detect intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1) and P-selectin using SERS. Results: SERS and BFNP were utilized to detect, discriminate and quantify ICAM-1, VCAM-1 and P-selectin in vitro on human endothelial cells and ex vivo in human coronary arteries. Ultimately, non-invasive multiplex imaging of adhesion molecules in a humanized mouse model was demonstrated in vivo following intravenous injection of the nanoprobes. Conclusion: This study demonstrates that multiplexed SERS-based molecular imaging can indicate the status of vascular inflammation in vivo and gives promise for SERS as a clinical imaging technique for cardiovascular disease in the future.
Issue Date: 1-Jan-2018
Date of Acceptance: 18-Oct-2018
URI: http://hdl.handle.net/10044/1/66127
DOI: https://dx.doi.org/10.7150/thno.28665
ISSN: 1838-7640
Publisher: Ivyspring International Publisher
Start Page: 6195
End Page: 6209
Journal / Book Title: Theranostics
Volume: 8
Issue: 22
Copyright Statement: © 2018 The authors. This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Sponsor/Funder: Engineering & Physical Science Research Council (E
Funder's Grant Number: EP/L014165/1
Keywords: Science & Technology
Life Sciences & Biomedicine
Medicine, Research & Experimental
Research & Experimental Medicine
atherosclerosis
molecular imaging
multiplexing
vascular inflammation
surface-enhanced Raman spectroscopy (SERS)
APPLIED SERS NANOPARTICLES
INTRAOPERATIVE GUIDANCE
CARDIOVASCULAR-DISEASE
ENDOTHELIAL-CELLS
ATHEROSCLEROSIS
FRESH
MECHANISMS
NANOTAGS
IMMUNITY
PLAQUES
Publication Status: Published
Open Access location: http://www.thno.org/v08p6195.pdf
Online Publication Date: 2018-11-29
Appears in Collections:Mathematics
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