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Measuring bilayer surface energy and curvature in asymmetric droplet interface bilayers

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Title: Measuring bilayer surface energy and curvature in asymmetric droplet interface bilayers
Authors: Barlow, N
Kusumaatmaja, H
Salehi-Reyhani, A
Brooks, N
Barter, LMC
Flemming, AJ
Ces, O
Item Type: Journal Article
Abstract: For the past decade, droplet interface bilayers (DIBs) have had an increased prevalence in biomolecular and biophysical literature. However, much of the underlying physics of these platforms is poorly characterized. To further our understanding of these structures, lipid membrane tension on DIB membranes is measured by analysing the equilibrium shape of asymmetric DIBs. To this end, the morphology of DIBs is explored for the first time using confocal laser scanning fluorescence microscopy. The experimental results confirm that, in accordance with theory, the bilayer interface of a volume-asymmetric DIB is curved towards the smaller droplet and a lipid-asymmetric DIB is curved towards the droplet with the higher monolayer surface tension. Moreover, the DIB shape can be exploited to measure complex bilayer surface energies. In this study, the bilayer surface energy of DIBs composed of lipid mixtures of phosphatidylgylcerol (PG) and phosphatidylcholine are shown to increase linearly with PG concentrations up to 25%. The assumption that DIB bilayer area can be geometrically approximated as a spherical cap base is also tested, and it is discovered that the bilayer curvature is negligible for most practical symmetric or asymmetric DIB systems with respect to bilayer area.
Issue Date: 1-Nov-2018
Date of Acceptance: 23-Oct-2018
URI: http://hdl.handle.net/10044/1/65766
DOI: https://dx.doi.org/10.1098/rsif.2018.0610
ISSN: 1742-5662
Publisher: Royal Society, The
Journal / Book Title: Journal of the Royal Society Interface
Volume: 15
Issue: 148
Copyright Statement: © 2018 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
Sponsor/Funder: Commission of the European Communities
Biotechnology and Biological Sciences Research Council (BBSRC)
Funder's Grant Number: 607466
BB/SCA/Imperial/17
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
droplet interface bilayers
surface energy
membrane asymmetry
FLIP-FLOP
DYNAMIC MORPHOLOGIES
WATER PERMEABILITY
GIANT VESICLES
TENSION
MEMBRANE
ADSORPTION
LIPIDS
PHOSPHOLIPIDS
CAPACITANCE
MD Multidisciplinary
General Science & Technology
Publication Status: Published
Online Publication Date: 2018-11-21
Appears in Collections:Chemistry
Biological and Biophysical Chemistry
Faculty of Natural Sciences



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