The cyst-dividing bacterium ramlibacter tataouinensis TTB310 genome reveals a well-stocked toolbox for adaptation to a desert environment

Title: The cyst-dividing bacterium ramlibacter tataouinensis TTB310 genome reveals a well-stocked toolbox for adaptation to a desert environment
Authors: De Luca, G
Barakat, M
Ortet, P
Fochesato, S
Jourlin-Castelli, C
Ansaldi, M
Py, B
Fichant, G
Coutinho, PM
Voulhoux, R
Bastien, O
Marechal, E
Henrissat, B
Quentin, Y
Noirot, P
Filloux, A
Mejean, V
DuBow, MS
Barras, F
Barbe, V
Weissenbach, J
Mihalcescu, I
Vermeglio, A
Achouak, W
Heulin, T
Item Type: Journal Article
Publication Date: 2011-09-01
2019-01-16T09:51:58Z
Abstract: Ramlibacter tataouinensis TTB310T (strain TTB310), a betaproteobacterium isolated from a semi-arid region of South Tunisia (Tataouine), is characterized by the presence of both spherical and rod-shaped cells in pure culture. Cell division of strain TTB310 occurs by the binary fission of spherical “cyst-like” cells (“cyst-cyst” division). The rod-shaped cells formed at the periphery of a colony (consisting mainly of cysts) are highly motile and colonize a new environment, where they form a new colony by reversion to cyst-like cells. This unique cell cycle of strain TTB310, with desiccation tolerant cyst-like cells capable of division and desiccation sensitive motile rods capable of dissemination, appears to be a novel adaptation for life in a hot and dry desert environment. In order to gain insights into strain TTB310's underlying genetic repertoire and possible mechanisms responsible for its unusual lifestyle, the genome of strain TTB310 was completely sequenced and subsequently annotated. The complete genome consists of a single circular chromosome of 4,070,194 bp with an average G+C content of 70.0%, the highest among the Betaproteobacteria sequenced to date, with total of 3,899 predicted coding sequences covering 92% of the genome. We found that strain TTB310 has developed a highly complex network of two-component systems, which may utilize responses to light and perhaps a rudimentary circadian hourglass to anticipate water availability at the dew time in the middle/end of the desert winter nights and thus direct the growth window to cyclic water availability times. Other interesting features of the strain TTB310 genome that appear to be important for desiccation tolerance, including intermediary metabolism compounds such as trehalose or polyhydroxyalkanoate, and signal transduction pathways, are presented and discussed.
Issue Date: 1-Sep-2011
Date of Acceptance: 25-Jul-2011
URI: http://hdl.handle.net/10044/1/65711
DOI: https://dx.doi.org/10.1371/journal.pone.0023784
ISSN: 1932-6203
Publisher: Public Library of Science (PLoS)
Journal / Book Title: PLoS ONE
Volume: 6
Issue: 9
Copyright Statement: © 2011 De Luca et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
SIGNAL-TRANSDUCTION
CIRCADIAN CLOCK
TATAHOUINE METEORITE
STRUCTURAL INSIGHTS
TWITCHING MOTILITY
PROTEIN SECRETION
BIOFILM FORMATION
ESCHERICHIA-COLI
WATER CHANNEL
IV PILI
Adaptation, Physiological
Carbohydrate Metabolism
Cell Division
Cell Membrane
Cell Movement
Cell Shape
Circadian Rhythm
Comamonadaceae
DNA Repair
DNA, Bacterial
Desert Climate
Extracellular Space
Fatty Acids
Genome, Bacterial
Genomics
Hydrolysis
Light
Membrane Fluidity
Membrane Lipids
Osmotic Pressure
Oxidative Stress
Polysaccharides, Bacterial
Protein Transport
Sequence Analysis, DNA
Signal Transduction
Trehalose
Cell Membrane
Extracellular Space
Comamonadaceae
Trehalose
Polysaccharides, Bacterial
Fatty Acids
Membrane Lipids
DNA, Bacterial
Sequence Analysis, DNA
Genomics
Desert Climate
Adaptation, Physiological
Signal Transduction
Cell Division
Cell Movement
Cell Shape
DNA Repair
Membrane Fluidity
Protein Transport
Hydrolysis
Oxidative Stress
Circadian Rhythm
Genome, Bacterial
Osmotic Pressure
Light
Carbohydrate Metabolism
Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
SIGNAL-TRANSDUCTION
CIRCADIAN CLOCK
TATAHOUINE METEORITE
STRUCTURAL INSIGHTS
TWITCHING MOTILITY
PROTEIN SECRETION
BIOFILM FORMATION
ESCHERICHIA-COLI
WATER CHANNEL
IV PILI
MD Multidisciplinary
General Science & Technology
Publication Status: Published
Article Number: e23784
metadata.pubs.date.publish-online: 2011-09-01
Appears in Collections:Faculty of Natural Sciences



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