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Synthetic biology tools for engineering Yarrowia lipolytica

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Title: Synthetic biology tools for engineering Yarrowia lipolytica
Authors: Larroude, M
Rossignol, T
Nicaud, J-M
Ledesma Amaro, R
Item Type: Journal Article
Abstract: The non-conventional oleaginous yeast Yarrowia lipolytica shows great industrial promise. It naturally produces certain compounds of interest but can also artificially generate non-native metabolites, thanks to an engineering process made possible by the significant expansion of a dedicated genetic toolbox. In this review, we present recently developed synthetic biology tools that facilitate the manipulation of Y. lipolytica, including 1) DNA assembly techniques, 2) DNA parts for constructing expression cassettes, 3) genome-editing techniques, and 4) computational tools.
Issue Date: 1-Dec-2018
Date of Acceptance: 7-Oct-2018
URI: http://hdl.handle.net/10044/1/65509
DOI: https://dx.doi.org/10.1016/j.biotechadv.2018.10.004
ISSN: 0734-9750
Publisher: Elsevier
Start Page: 2150
End Page: 2164
Journal / Book Title: Biotechnology Advances
Volume: 36
Issue: 8
Copyright Statement: © 2018 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/BY/4.0/)
Sponsor/Funder: Biotechnology and Biological Sciences Research Council (BBSRC)
Funder's Grant Number: BB/R01602X/1
Keywords: Science & Technology
Life Sciences & Biomedicine
Biotechnology & Applied Microbiology
Yarrowia lipolytica
Synthetic biology
DNA assembly
CRISPR-Cas9
Genome editing
Golden Gate
Industrial biotechnology
Genome scale metabolic models
Metabolic engineering
OMEGA-3 EICOSAPENTAENOIC ACID
CITRIC-ACID
LIPID-ACCUMULATION
PROTEIN EXPRESSION
GENE-EXPRESSION
RAW GLYCEROL
HOMOLOGOUS RECOMBINATION
HETEROLOGOUS PROTEINS
HANSENULA-POLYMORPHA
ALPHA-KETOGLUTARATE
06 Biological Sciences
09 Engineering
10 Technology
Biotechnology
Publication Status: Published
Open Access location: https://www.sciencedirect.com/science/article/pii/S0734975018301678#f0010
Online Publication Date: 2018-10-11
Appears in Collections:Bioengineering



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