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IMR90 ER:RAS: A cell model of oncogene-induced senescence

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Title: IMR90 ER:RAS: A cell model of oncogene-induced senescence
Authors: Innes, AJ
Gil, J
Item Type: Journal Article
Abstract: Oncogene-induced senescence (OIS) is a cellular response that limits the replication of cells expressing oncogenes. As a result, OIS is a potent tumor suppressor mechanism limiting cancer progression. Here we describe IMR90 ER:RAS, a widely used model to study OIS in cell culture. This model takes advantage of IMR90 human primary fibroblast infected with a 4-hydroxy-tamoxifen (4-OHT) inducible ER:RAS construct. RAS activation upon 4-OHT treatment results in a coordinated induction of senescence, recapitulating different aspects of the phenotype such as the growth arrest and the establishment of a senescence-associated secretory phenotype (SASP).
Issue Date: 31-Jan-2019
Date of Acceptance: 1-Jan-2019
URI: http://hdl.handle.net/10044/1/65506
DOI: https://dx.doi.org/10.1007/978-1-4939-8931-7_9
ISSN: 1940-6029
Publisher: Humana Press
Start Page: 83
End Page: 92
Journal / Book Title: Methods in Molecular Biology
Volume: 1896
Copyright Statement: © 2019 Springer Science+Business Media, LLC, part of Springer Nature. The final publication is available at Springer via https://dx.doi.org/10.1007/978-1-4939-8931-7_9
Keywords: BrdU
Growth arrest
Oncogene-induced senescence
SASP
Senescence
p16INK4a
p21CIP1
p53
BrdU
Growth arrest
Oncogene-induced senescence
SASP
Senescence
p16INK4a
p21CIP1
p53
0601 Biochemistry And Cell Biology
Developmental Biology
Publication Status: Published
Conference Place: United States
Embargo Date: 2020-01-01
Appears in Collections:Clinical Sciences
Molecular Sciences
Department of Medicine
Faculty of Medicine



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