The Metabolic Fate and Effects of 2-Bromophenol in Male Sprague-Dawley Rats.

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Title: The Metabolic Fate and Effects of 2-Bromophenol in Male Sprague-Dawley Rats.
Authors: Adesina-Georgiadis, KN
Gray, N
Plumb, RS
Thompson, DF
Holmes, E
Nicholson, JK
Wilson, ID
Item Type: Journal Article
Abstract: 1. The metabolic fate and urinary excretion of 2-bromophenol, a phenolic metabolite of bromobenzene, was investigated in male Sprague Dawley rats following single intraperitoneal doses at either 0, 100 or 200 mg/kg. 2. Urine was collected for seven days and samples analysed using 1H NMR spectroscopy, inductively coupled plasma (ICP)MS, and UPLC-MS. 3. 1H NMR spectroscopy of the urine samples showed that, at these doses, 2-bromophenol had little effect on endogenous metabolite profiles, supporting histopathology and clinical chemistry data which showed no changes associated with the administration of 2-bromophenol at these doses. 4. The use of ICP-MS Provided a means for the selective detection and quantification of bromine-containing species and showed that between 15 and 30% of the dose was excreted via the urine over the 7 days of the study for both the 100 and 200 mg doses respectively. 6. The bulk of the excretion of Br-containing material had occurred by 8 hr post administration. UPLC-MS of urine revealed a number of metabolites of 2-bromophenol, with 2-bromophenol glucuronide and 2-bromophenol sulphate identified as the major species. A number of minor hydroxylated metabolites were also detected as their glucuronide, sulphate or O-methyl conjugates. There was no evidence for the production of reactive metabolites.
Issue Date: 17-Dec-2018
URI: http://hdl.handle.net/10044/1/65434
DOI: https://dx.doi.org/10.1080/00498254.2018.1559376
Start Page: 1
End Page: 22
Journal / Book Title: Xenobiotica
Copyright Statement: © 2018 Taylor & Francis. This is an Accepted Manuscript of an article published by Taylor & Francis in Xenobiotica on 17 Dec 2018, available online: https://dx.doi.org/10.1080/00498254.2018.1559376
Keywords: . 2-Bromobenzene
metabolism
metabonomics
nephrotoxicity
0601 Biochemistry And Cell Biology
1115 Pharmacology And Pharmaceutical Sciences
Pharmacology & Pharmacy
Publication Status: Published online
Conference Place: England
Embargo Date: 2019-12-17
Appears in Collections:Division of Surgery
Faculty of Medicine



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